Team:Groningen/Notebook/9 July 2009

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Wet

Purification of HmtA (PA Q9I147 6xHis Tag)

  • SDS-gel electrophosis
  • Make a 12% SDS-gel
  • Fill gel with samples Wash 1-2 20mM imidazole, Wash1-2 40mM imidazole, Elute1-3 and ladders
  • Run 2h at 100V
  • Stain the gel in coomassie blue.


Inoculation of TY-medium for Glycerol Stocks and Plasmid isolation

  • BBa_J23109 (labelled as no.1) vector BBa_J61002 in E.coli TOP10
  • BBa_J23100 (labelled as no.2) vector BBa_J61002 in E.coli TOP10 (red colony)
  • BBa_J23106 (labelled as no.3) vector BBa_J61002 in E.coli TOP10 (red colony)
  • The three plates showing new colonies were used to inoculate 5mL TY-medium with amp. (final conc. 100mg/ul).
  • The tubes were put in a waterbath at 37C (200 rpm) and grown o.n.



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