Team:Groningen/Parts

From 2009.igem.org

(Difference between revisions)
(Part List -80)
(Part List -80)
Line 24: Line 24:
|'''Resistance'''
|'''Resistance'''
|'''Well'''
|'''Well'''
 +
|'''Quality control'''
|-
|-
|GVP
|GVP
Line 32: Line 33:
|Ampicillin/Gentamycin
|Ampicillin/Gentamycin
|plate 3, pos.1
|plate 3, pos.1
 +
|?
|-
|-
|GVP (2nd batch)
|GVP (2nd batch)
Line 40: Line 42:
|Ampicillin/Gentamycin
|Ampicillin/Gentamycin
|plate 3, pos.2
|plate 3, pos.2
 +
|?
|-
|-
-
|(?)pHigh in low copy nr vector
+
|pHigh in low copy nr vector
|High consitutive promoter
|High consitutive promoter
|<partinfo>J23100</partinfo>
|<partinfo>J23100</partinfo>
Line 48: Line 51:
|Kanamycin
|Kanamycin
|Plate 3, pos. 4
|Plate 3, pos. 4
 +
|PCR(?), restriction(+)
|-
|-
-
|(?)pMed in low copy nr vector
+
|pMed in low copy nr vector
|Medium consitutive promoter
|Medium consitutive promoter
|<partinfo>J23106</partinfo>
|<partinfo>J23106</partinfo>
Line 56: Line 60:
|Kanamycin
|Kanamycin
|Plate 3, pos. 5
|Plate 3, pos. 5
 +
|PCR(?), restriction(+)
|-
|-
-
|(?)pLow in low copy nr vector
+
|pLow in low copy nr vector
|Low consitutive promoter
|Low consitutive promoter
|<partinfo>J23109</partinfo>
|<partinfo>J23109</partinfo>
Line 64: Line 69:
|Kanamycin
|Kanamycin
|Plate 3, pos. 6
|Plate 3, pos. 6
 +
|PCR(?), restriction(+)
|-
|-
-
|(?)pHigh in high copy nr vector
+
|pHigh in high copy nr vector
|High consitutive promoter
|High consitutive promoter
|<partinfo>J23100</partinfo>
|<partinfo>J23100</partinfo>
Line 72: Line 78:
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|Plate 3, pos. 7
|Plate 3, pos. 7
 +
|PCR(?), restriction(+)
|-
|-
-
|(?)pMed in high copy nr vector
+
|pMed in high copy nr vector
|Medium consitutive promoter
|Medium consitutive promoter
|<partinfo>J23106</partinfo>
|<partinfo>J23106</partinfo>
Line 80: Line 87:
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|Plate 3, pos. 8
|Plate 3, pos. 8
 +
|PCR(?), restriction(+)
|-
|-
-
|(?)pLow in high copy nr vector
+
|pLow in high copy nr vector
|High consitutive promoter
|High consitutive promoter
|<partinfo>J23109</partinfo>
|<partinfo>J23109</partinfo>
Line 88: Line 96:
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|Plate 3, pos. 9
|Plate 3, pos. 9
 +
|PCR(?), restriction(+)
|-
|-
|Promotor (low expression)
|Promotor (low expression)
Line 96: Line 105:
|Ampicillin
|Ampicillin
|plate 3, pos.10
|plate 3, pos.10
 +
|No
|-
|-
|Promotor (medium expression)
|Promotor (medium expression)
Line 104: Line 114:
|Ampicillin
|Ampicillin
|plate 3, pos.11
|plate 3, pos.11
 +
|No
|-
|-
|Promotor (high expression)
|Promotor (high expression)
Line 112: Line 123:
|Ampicillin
|Ampicillin
|plate 3, pos.12
|plate 3, pos.12
 +
|No
|-
|-
|Inducible promoter
|Inducible promoter
Line 120: Line 132:
|Ampicillin
|Ampicillin
|Plate 3, pos. 14
|Plate 3, pos. 14
 +
|PCR(?), restriction(+)
|-
|-
|Inducible promoter
|Inducible promoter
Line 128: Line 141:
|Ampicillin
|Ampicillin
|Plate 3, pos. 15
|Plate 3, pos. 15
 +
|PCR(?), restriction(+)
|-
|-
|RBS
|RBS
Line 136: Line 150:
|Ampicillin
|Ampicillin
|plate 3, pos.19
|plate 3, pos.19
 +
|No
|-  
|-  
|Terminator
|Terminator
Line 144: Line 159:
|Ampicillin/Kanamycin
|Ampicillin/Kanamycin
|plate 3, pos.20
|plate 3, pos.20
 +
|No
|-  
|-  
|HmtA (1.1)
|HmtA (1.1)
-
|?
+
|HmtA-His in pBAD(a)
|None
|None
|?
|?
Line 152: Line 168:
|Ampicillin
|Ampicillin
|plate 3, pos.28
|plate 3, pos.28
 +
|Restriction(+)
|-  
|-  
|HmtA (2.1)
|HmtA (2.1)
-
|?
+
|HmtA-His in pBAD(a)
|None
|None
|?
|?
Line 160: Line 177:
|Ampicillin
|Ampicillin
|plate 3, pos.29
|plate 3, pos.29
 +
|Restriction(+)
|}
|}

Revision as of 13:50, 4 August 2009

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Part List -80

Component Description Part or Accession # Base Pairs (bp) Plasmid (backbone) Resistance Well Quality control
GVP Gas Vesicle Proteins BBa_I750016 6064 BBa_J61035 Ampicillin/Gentamycin plate 3, pos.1 ?
GVP (2nd batch) Gas Vesicle Proteins BBa_I750016 6064 BBa_J61035 Ampicillin/Gentamycin plate 3, pos.2 ?
pHigh in low copy nr vector High consitutive promoter 35bp Kanamycin Plate 3, pos. 4 PCR(?), restriction(+)
pMed in low copy nr vector Medium consitutive promoter 35bp Kanamycin Plate 3, pos. 5 PCR(?), restriction(+)
pLow in low copy nr vector Low consitutive promoter 35bp Kanamycin Plate 3, pos. 6 PCR(?), restriction(+)
pHigh in high copy nr vector High consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 7 PCR(?), restriction(+)
pMed in high copy nr vector Medium consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 8 PCR(?), restriction(+)
pLow in high copy nr vector High consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 9 PCR(?), restriction(+)
Promotor (low expression) Constitutive promotor BBa_J23109 35 BBa_J61002 Ampicillin plate 3, pos.10 No
Promotor (medium expression) Constitutive promotor BBa_J23106 35 BBa_J61002 Ampicillin plate 3, pos.11 No
Promotor (high expression) Constitutive promotor BBa_J23100 35 BBa_J61002 Ampicillin plate 3, pos.12 No
Inducible promoter Lactose inducible promoter nr1 200bp Ampicillin Plate 3, pos. 14 PCR(?), restriction(+)
Inducible promoter Lactose inducible promoter nr2 200bp Ampicillin Plate 3, pos. 15 PCR(?), restriction(+)
RBS Ribosomal Binding Site BBa_B0034 12 pSB1A2 Ampicillin plate 3, pos.19 No
Terminator Double terminator consisting of BBa_B0012 and BBa_B0011 BBa_B0014 ~90 pSB1AK3 Ampicillin/Kanamycin plate 3, pos.20 No
HmtA (1.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.28 Restriction(+)
HmtA (2.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.29 Restriction(+)