Team:Groningen/Parts

From 2009.igem.org

(Difference between revisions)
m
Line 45: Line 45:
|High consitutive promoter
|High consitutive promoter
|<partinfo>J23100</partinfo>
|<partinfo>J23100</partinfo>
-
|35bp
+
|35
|<partinfo>pSB3K3</partinfo>  
|<partinfo>pSB3K3</partinfo>  
|Kanamycin
|Kanamycin
Line 54: Line 54:
|Medium consitutive promoter
|Medium consitutive promoter
|<partinfo>J23106</partinfo>
|<partinfo>J23106</partinfo>
-
|35bp
+
|35
|<partinfo>pSB3K3</partinfo>  
|<partinfo>pSB3K3</partinfo>  
|Kanamycin
|Kanamycin
Line 63: Line 63:
|Low consitutive promoter
|Low consitutive promoter
|<partinfo>J23109</partinfo>
|<partinfo>J23109</partinfo>
-
|35bp
+
|35
|<partinfo>pSB3K3</partinfo>  
|<partinfo>pSB3K3</partinfo>  
|Kanamycin
|Kanamycin
Line 72: Line 72:
|High consitutive promoter
|High consitutive promoter
|<partinfo>J23100</partinfo>
|<partinfo>J23100</partinfo>
-
|35bp
+
|35
|<partinfo>pSB1AC3</partinfo>  
|<partinfo>pSB1AC3</partinfo>  
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
Line 81: Line 81:
|Medium consitutive promoter
|Medium consitutive promoter
|<partinfo>J23106</partinfo>
|<partinfo>J23106</partinfo>
-
|35bp
+
|35
|<partinfo>pSB1AC3</partinfo>  
|<partinfo>pSB1AC3</partinfo>  
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
Line 90: Line 90:
|High consitutive promoter
|High consitutive promoter
|<partinfo>J23109</partinfo>
|<partinfo>J23109</partinfo>
-
|35bp
+
|35
|<partinfo>pSB1AC3</partinfo>  
|<partinfo>pSB1AC3</partinfo>  
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
Line 126: Line 126:
|Lactose inducible promoter nr1
|Lactose inducible promoter nr1
|<partinfo>R0010</partinfo>
|<partinfo>R0010</partinfo>
-
|200bp
+
|200
|<partinfo>pSB1A2</partinfo>  
|<partinfo>pSB1A2</partinfo>  
|Ampicillin
|Ampicillin
Line 135: Line 135:
|Lactose inducible promoter nr2
|Lactose inducible promoter nr2
|<partinfo>R0010</partinfo>
|<partinfo>R0010</partinfo>
-
|200bp
+
|200
|<partinfo>pSB1A2</partinfo>  
|<partinfo>pSB1A2</partinfo>  
|Ampicillin
|Ampicillin
Line 289: Line 289:
|<partinfo>BBa_K190024</partinfo>
|<partinfo>BBa_K190024</partinfo>
|71
|71
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between EcoRI and SpeI)
|Ampicillin
|Ampicillin
|plate 3, pos.45
|plate 3, pos.45
Line 297: Line 297:
|SmtA-GST fusion  
|SmtA-GST fusion  
|no biobrick primers available
|no biobrick primers available
-
|1400bp
+
|1400
-
|pGEX-3x (NEB)
+
|[http://www.ecoliwiki.net/colipedia/index.php/pGEX-3X pGEX-3x]
|Ampicillin
|Ampicillin
|plate 3, pos. 46
|plate 3, pos. 46
Line 306: Line 306:
|SmtA
|SmtA
|<partinfo>BBa_K190021</partinfo>
|<partinfo>BBa_K190021</partinfo>
-
|900bp
+
|900
-
|pET29a (NEB)
+
|[http://www.merckbiosciences.co.uk/product/69871 pET29a]
|Kanamycin
|Kanamycin
|plate 3, pos. 47
|plate 3, pos. 47
Line 315: Line 315:
|MymT
|MymT
|<partinfo>BBa_K190020</partinfo>
|<partinfo>BBa_K190020</partinfo>
-
|215bp
+
|215
 +
|[http://www.neb.com/nebecomm/products/productN6707.asp pTXB1] (NEB)
|Ampicillin
|Ampicillin
-
|pTXB1 (NEB)
 
|plate 3, pos. 48
|plate 3, pos. 48
|PCR, restriction
|PCR, restriction
Line 324: Line 324:
|GlpF and fMT on a synthetic operon in the vector
|GlpF and fMT on a synthetic operon in the vector
|<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo>
|<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo>
-
|fMT = 200bp, GlpF = 900bp?
+
|fMT = 200, GlpF = 900?
 +
|[http://www.neb.com/nebecomm/products/productN8076.asp pMALc2x] (NEB)
|Ampicillin
|Ampicillin
-
|pMALc2x (NEB)
 
|plate 3, pos. 49
|plate 3, pos. 49
|PCR, restriction
|PCR, restriction
Line 333: Line 333:
|MBP-ArsR Fusion protein
|MBP-ArsR Fusion protein
|<partinfo>BBa_K190027</partinfo>
|<partinfo>BBa_K190027</partinfo>
-
|15180 bp
+
|15180
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 342: Line 342:
|GVP Gene cluster
|GVP Gene cluster
|{{todo|}}
|{{todo|}}
-
|6000 bp
+
|6000
-
|pBluescriptIIKS
+
|[http://www.fermentas.com/techinfo/nucleicacids/mappbluescriptiiskks.htm pBluescriptIIKS]
|{{todo| Ampicillin}}
|{{todo| Ampicillin}}
|plate 3, pos. 54
|plate 3, pos. 54
Line 351: Line 351:
|Metallothionein with inducible promotor
|Metallothionein with inducible promotor
|<partinfo>BBa_K190032</partinfo>
|<partinfo>BBa_K190032</partinfo>
-
|430 bp
+
|430
|<partinfo>pSB1A2</partinfo>
|<partinfo>pSB1A2</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 360: Line 360:
|Metallothionein with low constitutive promotor
|Metallothionein with low constitutive promotor
|<partinfo>BBa_K190031</partinfo>
|<partinfo>BBa_K190031</partinfo>
-
|265 bp
+
|265
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 369: Line 369:
|Metallothionein with low constitutive promotor
|Metallothionein with low constitutive promotor
|<partinfo>BBa_K190031</partinfo>
|<partinfo>BBa_K190031</partinfo>
-
|265 bp
+
|265  
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 378: Line 378:
|Metallothionein
|Metallothionein
|<partinfo>BBa_K190021</partinfo>
|<partinfo>BBa_K190021</partinfo>
-
|678 bp
+
|678
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 387: Line 387:
|Metallothionein for As<sup>3+</sup>
|Metallothionein for As<sup>3+</sup>
|<partinfo>BBa_K190019</partinfo>
|<partinfo>BBa_K190019</partinfo>
-
|222 bp
+
|222
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 396: Line 396:
|Metallothionein for As<sup>3+</sup>
|Metallothionein for As<sup>3+</sup>
|<partinfo>BBa_K190019</partinfo>
|<partinfo>BBa_K190019</partinfo>
-
| 222 bp
+
| 222
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol

Revision as of 08:31, 4 September 2009

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Biobricks


Note

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Part List -80

Component Description Part or Accession # Base Pairs (bp) Plasmid (backbone) Resistance Well Quality control
GVP Gas Vesicle Proteins 6064 Ampicillin/Gentamycin plate 3, pos.1 ?
GVP (2nd batch) Gas Vesicle Proteins 6064 Ampicillin/Gentamycin plate 3, pos.2 ?
pHigh in low copy nr vector High consitutive promoter 35 Kanamycin Plate 3, pos. 4 PCR(?), restriction(+)
pMed in low copy nr vector Medium consitutive promoter 35 Kanamycin Plate 3, pos. 5 PCR(?), restriction(+)
pLow in low copy nr vector Low consitutive promoter 35 Kanamycin Plate 3, pos. 6 PCR(?), restriction(+)
pHigh in high copy nr vector High consitutive promoter 35 Ampicillin/Chloramphenicol Plate 3, pos. 7 PCR(?), restriction(+)
pMed in high copy nr vector Medium consitutive promoter 35 Ampicillin/Chloramphenicol Plate 3, pos. 8 PCR(?), restriction(+)
pLow in high copy nr vector High consitutive promoter 35 Ampicillin/Chloramphenicol Plate 3, pos. 9 PCR(?), restriction(+)
Promotor (low expression) Constitutive promotor 35 Ampicillin plate 3, pos.10 No
Promotor (medium expression) Constitutive promotor 35 Ampicillin plate 3, pos.11 No
Promotor (high expression) Constitutive promotor 35 Ampicillin plate 3, pos.12 No
Inducible promoter Lactose inducible promoter nr1 200 Ampicillin Plate 3, pos. 14 PCR(?), restriction(+)
Inducible promoter Lactose inducible promoter nr2 200 Ampicillin Plate 3, pos. 15 PCR(?), restriction(+)
RBS Ribosomal Binding Site 12 Ampicillin plate 3, pos.19 No
Terminator Double terminator consisting of and ] ~90 Ampicillin/Kanamycin plate 3, pos.20 No
Const. Pormoter + GVP Low constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.21 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP (no.2) Low constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.22 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP Medium constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.23 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP (no.2) Medium constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.24 EcoRI/PstI + Sequenced Okay
HmtA (1.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.28 Restriction(+)
HmtA (2.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.29 Restriction(+)
CueO- + GVP in J61035 CueO promoter without RBS in front of the GVP cluster None 6149 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.37 Restriction(+) + Sequenced Okay
ZntR- + GVP in J61035 ZntR promoter without RBS in front of the GVP cluster None ~6150 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.38 Restriction(+) + Sequenced (3 mutations)
ArsR- + GVP in J61035 ArsR promoter without RBS in front of the GVP cluster None ~6150 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.39 Restriction(+) + Sequenced Okay
ArsR- in J61002 pArsR (promoter without RBS) 71 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.40 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR- in J61002 pZntR (promoter without RBS) 65 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.41 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations)
CueO- in J61002 pCueO (promoter without RBS) 43 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.42 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR+RBS in J61002 pZntR (promoter with own RBS) 91 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.43 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ArsR+RBS in J61002 pArsR (promoter with own RBS) 81 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.44 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
CueO+RBS in J61002 pCueO (promoter with own RBS) 71 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.45 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
SmtA-GST in pGEX-3x SmtA-GST fusion no biobrick primers available 1400 pGEX-3x Ampicillin plate 3, pos. 46 PCR, restriction
SmtA in pET29a SmtA 900 pET29a Kanamycin plate 3, pos. 47 PCR, restriction
MymT in pGB68 MymT 215 pTXB1 (NEB) Ampicillin plate 3, pos. 48 PCR, restriction
GlpF and fMT in pMAL-MT GlpF and fMT on a synthetic operon in the vector and fMT = 200, GlpF = 900? pMALc2x (NEB) Ampicillin plate 3, pos. 49 PCR, restriction
MBP-ArsR MBP-ArsR Fusion protein 15180 Ampicillin/chloramphenicol plate 3, pos. 53 Restriction analysis] & PCR
pNL29 GVP Gene cluster 6000 pBluescriptIIKS Ampicillin plate 3, pos. 54 Buoyancy phenotype
pLac-fMT Metallothionein with inducible promotor 430 Ampicillin/chloramphenicol plate 3, pos. 55 Quality control
pLow-fMT #4 Metallothionein with low constitutive promotor 265 Ampicillin/chloramphenicol plate 3, pos. 56 Quality control
pLow-fMT #3 Metallothionein with low constitutive promotor 265 Ampicillin/chloramphenicol plate 3, pos. 57 Quality control
SmtA Metallothionein 678 Ampicillin/chloramphenicol plate 3, pos. 58 Quality control
fMT #6 Metallothionein for As3+ 222 Ampicillin/chloramphenicol plate 3, pos. 59 Sequence 2 deletions between EcoRI and XbaI sites
fMT #4 Metallothionein for As3+ 222 Ampicillin/chloramphenicol plate 3, pos. 60 Sequence Okay!
High consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Ampicillin/chloramphenicol plate 3, pos. 75 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Ampicillin/chloramphenicol plate 3, pos. 76 Quality control
Low consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Ampicillin/chloramphenicol plate 3, pos. 77 Quality control
High consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Kanamycin plate 3, pos. 78 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Kanamycin plate 3, pos. 79 Quality control
Low consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Kanamycin plate 3, pos. 80 Quality control
Reference constitutive promoter) Constitutive promoter ? Base Pairs (bp) ? ? plate 3, pos. 81 Quality control