Team:Groningen/Parts

From 2009.igem.org

(Difference between revisions)
(Part List -80)
(Part List -80)
Line 239: Line 239:
|plate 3, pos.39
|plate 3, pos.39
|Restriction(+)
|Restriction(+)
 +
|-
 +
|ArsR- in J61002
 +
|pArsR (promoter without RBS)
 +
|BBa_K190015
 +
|71
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
 +
|Ampicillin
 +
|plate 3, pos.40
 +
|EcoRI/SpeI(+), EcoRI/PstI(+)
 +
|-
 +
|ZntR- in J61002
 +
|pZntR (promoter without RBS)
 +
|BBa_K190016
 +
|65
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
 +
|Ampicillin
 +
|plate 3, pos.41
 +
|EcoRI/SpeI(+), EcoRI/PstI(+)
 +
|-
 +
|CueO- in J61002
 +
|pCueO (promoter without RBS)
 +
|BBa_K190017
 +
|43
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
 +
|Ampicillin
 +
|plate 3, pos.42
 +
|EcoRI/SpeI(+), EcoRI/PstI(+)
|}
|}

Revision as of 10:19, 21 August 2009

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Part List -80

Component Description Part or Accession # Base Pairs (bp) Plasmid (backbone) Resistance Well Quality control
GVP Gas Vesicle Proteins BBa_I750016 6064 BBa_J61035 Ampicillin/Gentamycin plate 3, pos.1 ?
GVP (2nd batch) Gas Vesicle Proteins BBa_I750016 6064 BBa_J61035 Ampicillin/Gentamycin plate 3, pos.2 ?
pHigh in low copy nr vector High consitutive promoter 35bp Kanamycin Plate 3, pos. 4 PCR(?), restriction(+)
pMed in low copy nr vector Medium consitutive promoter 35bp Kanamycin Plate 3, pos. 5 PCR(?), restriction(+)
pLow in low copy nr vector Low consitutive promoter 35bp Kanamycin Plate 3, pos. 6 PCR(?), restriction(+)
pHigh in high copy nr vector High consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 7 PCR(?), restriction(+)
pMed in high copy nr vector Medium consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 8 PCR(?), restriction(+)
pLow in high copy nr vector High consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 9 PCR(?), restriction(+)
Promotor (low expression) Constitutive promotor BBa_J23109 35 BBa_J61002 Ampicillin plate 3, pos.10 No
Promotor (medium expression) Constitutive promotor BBa_J23106 35 BBa_J61002 Ampicillin plate 3, pos.11 No
Promotor (high expression) Constitutive promotor BBa_J23100 35 BBa_J61002 Ampicillin plate 3, pos.12 No
Inducible promoter Lactose inducible promoter nr1 200bp Ampicillin Plate 3, pos. 14 PCR(?), restriction(+)
Inducible promoter Lactose inducible promoter nr2 200bp Ampicillin Plate 3, pos. 15 PCR(?), restriction(+)
RBS Ribosomal Binding Site BBa_B0034 12 pSB1A2 Ampicillin plate 3, pos.19 No
Terminator Double terminator consisting of BBa_B0012 and BBa_B0011 BBa_B0014 ~90 pSB1AK3 Ampicillin/Kanamycin plate 3, pos.20 No
Const. Pormoter + GVP Low constitutive promoter with GVP cluster BBa_J23109 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.21 EcoRI/PstI
Const. Pormoter + GVP (no.2) Low constitutive promoter with GVP cluster BBa_J23109 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.22 EcoRI/PstI
Const. Pormoter + GVP Medium constitutive promoter with GVP cluster BBa_J23106 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.23 EcoRI/PstI
Const. Pormoter + GVP (no.2) Medium constitutive promoter with GVP cluster BBa_J23106 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.24 EcoRI/PstI
HmtA (1.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.28 Restriction(+)
HmtA (2.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.29 Restriction(+)
CueO- + GVP in J61035 CueO promoter without RBS in front of the GVP cluster None 6149 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.37 Restriction(+)
ZntR- + GVP in J61035 ZntR promoter without RBS in front of the GVP cluster None ~6150 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.38 Restriction(+)
ArsR- + GVP in J61035 ArsR promoter without RBS in front of the GVP cluster None ~6150 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.39 Restriction(+)
ArsR- in J61002 pArsR (promoter without RBS) BBa_K190015 71 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.40 EcoRI/SpeI(+), EcoRI/PstI(+)
ZntR- in J61002 pZntR (promoter without RBS) BBa_K190016 65 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.41 EcoRI/SpeI(+), EcoRI/PstI(+)
CueO- in J61002 pCueO (promoter without RBS) BBa_K190017 43 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.42 EcoRI/SpeI(+), EcoRI/PstI(+)