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Team:Groningen/Promoters
From 2009.igem.org
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This page shows the measurements we did of our constitutive promoters and derives approximate RPUs relative to the weakest promoter we use. For briefness the lines in the graphs use the original numbering, which can be summarized as follows:
| Plasmid/Part → | pSB1AC3 | pSB3K3 | BBa_J23109 | BBa_J23106 | BBa_J23100 | control | |||||||
| Medium → | LB | TY | LB | TY | LB | TY | LB | TY | LB | TY | LB | TY | |
| Dilution (of culture) ↓ | 1 | 7 | 2 | 8 | 3 | 9 | 4 | 10 | 5 | 11 | 6 | 12 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 100x | A | ||||||||||||
| B | |||||||||||||
| 200x | C | ||||||||||||
| D | |||||||||||||
| 400x | E | ||||||||||||
| F | |||||||||||||
Background
The following graphs show the optical density and fluorescence measurements for the controls. As can be seen 4 of the LB medium controls were probably contaminated, so these are ignored in the rest of the computations.
| LB controls | TY controls |
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Based on the non-contaminated control measurements we find the following average background values:
- LB
- OD600: 0.094262500378219 (A, F)
- Fluorescence: -11.4090909090909 (A)
- Fluorescence: -20.6136363636364 (B,C,D,E,F)
- TY
- OD600: 0.09115113622763 (A-F)
- Fluorescence: -9.06818181818182 (A)
- Fluorescence: -20.05 (B,C,D,E,F)
Using the above background values we get the graphs below.
| BBa_J23109 | BBa_J23106 | BBa_J23100 | |
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| TY | Loading graph...
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| LB | Loading graph...
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| TY | Loading graph...
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