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Team:HKU-HKBU/Parts
From 2009.igem.org
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=Parts submitted to the Registry= | =Parts submitted to the Registry= | ||
| - | '''This year, we constructed and submitted to the Registry a total of 42 new standard biobricks with code | + | '''This year, we constructed 50 and submitted to the Registry a total of 42 new standard biobricks with code numbers from K283000 to K283049. Please click [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=HKU-HKBU Biobricks from HKU-HKBU 2009] for their detailed information. Of these biobricks, XX contain complete information of primary nucleaic acid sequence, description of function, authorship, safety notes, acknowedgment of sources and references; TWO (BBa_K283003 and BBa_K283005) are the improved versions of the existing biobricks, BBa_K094102 and BBa_J36846, rescpectively; THIRTY-THREE (see Registry for more information) were demonstrated work as expected in our system, including SEVEN devices; seventeen left were not tested''' |
| - | After constructed a serial of intermediates, such as BBa_K283003, BBa_K283006, BBa_K283030, and so on, we eventually generated | + | After constructed a serial of intermediates, such as BBa_K283003, BBa_K283006, BBa_K283030, and so on, we eventually generated 4 '''USEFUL and FUNCTIONAL''' devices (BBa_K283000, BBa_K283001, BBa_K283002, BBa_K283014), in terms of polar expression system and speed controlling system. BBa_K283000 and BBa_K283001 were designed to display eGFP-streptavidin fusion protein on the cell surface with the help of a signal peptide of lipoprotein (''lpp'') and five transmembrane domains of outer membrane protein A (''ompA'') |
BBa_K283000 and BBa_K283002 are genetic circuits for polar expression and speed control respectively. | BBa_K283000 and BBa_K283002 are genetic circuits for polar expression and speed control respectively. | ||
Revision as of 14:32, 21 October 2009
Parts submitted to the Registry
This year, we constructed 50 and submitted to the Registry a total of 42 new standard biobricks with code numbers from K283000 to K283049. Please click Biobricks from HKU-HKBU 2009 for their detailed information. Of these biobricks, XX contain complete information of primary nucleaic acid sequence, description of function, authorship, safety notes, acknowedgment of sources and references; TWO (BBa_K283003 and BBa_K283005) are the improved versions of the existing biobricks, BBa_K094102 and BBa_J36846, rescpectively; THIRTY-THREE (see Registry for more information) were demonstrated work as expected in our system, including SEVEN devices; seventeen left were not tested
After constructed a serial of intermediates, such as BBa_K283003, BBa_K283006, BBa_K283030, and so on, we eventually generated 4 USEFUL and FUNCTIONAL devices (BBa_K283000, BBa_K283001, BBa_K283002, BBa_K283014), in terms of polar expression system and speed controlling system. BBa_K283000 and BBa_K283001 were designed to display eGFP-streptavidin fusion protein on the cell surface with the help of a signal peptide of lipoprotein (lpp) and five transmembrane domains of outer membrane protein A (ompA)
BBa_K283000 and BBa_K283002 are genetic circuits for polar expression and speed control respectively.
Figure 1. Assembly route of BBa_K283000
Figure 2. Assembly route of BBa_K283002
