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Parts submitted to the Registry

This year, we constructed 50 and submitted to the Registry a total of 42 new standard biobricks with code numbers from K283000 to K283049. Please click Biobricks from HKU-HKBU 2009 for their detailed information. Of these biobricks, XX contain complete information of primary nucleaic acid sequence, description of function, authorship, safety notes, acknowedgment of sources and references; TWO (BBa_K283003 and BBa_K283005) are the improved versions of the existing biobricks, BBa_K094102 and BBa_J36846, rescpectively; THIRTY-THREE (see Registry for more information) were demonstrated work as expected in our system, including SEVEN devices; seventeen left were not tested

After constructed a serial of intermediates, such as BBa_K283003, BBa_K283006, BBa_K283030, and so on, we eventually generated 4 USEFUL and FUNCTIONAL devices (BBa_K283000, BBa_K283001, BBa_K283002, BBa_K283014), in terms of polar expression system and speed controlling system. BBa_K283000 and BBa_K283001 were designed to display eGFP-streptavidin fusion protein on the cell surface with the help of a signal peptide of lipoprotein (lpp) and five transmembrane domains of outer membrane protein A (ompA)

BBa_K283000 and BBa_K283002 are genetic circuits for polar expression and speed control respectively.

Figure 1. Assembly route of BBa_K283000

Figure 2. Assembly route of BBa_K283002