Team:HKU-HKBU/motor results
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=Motor - Results= | =Motor - Results= | ||
- | ==Step 1== | + | |
+ | ==Motor Production== | ||
+ | |||
+ | ===Step 1 - Photoresist (SU-8) Spin Coating=== | ||
+ | [[Image:HKU-HKBU_motor_production_1.png | center]] | ||
+ | |||
+ | Note: | ||
+ | # Typical contaminants that must be removed prior to photoresist (SU-8) coating. | ||
+ | # Adhesion promoters are used to assist resist coating. | ||
+ | # Ideally want no H2O on wafer surface. | ||
+ | # Wafer is held on a spinner chuck by vacuum and resist is coated to uniform thickness by spin coating. | ||
+ | # Resist thickness is 1-2 mm. | ||
+ | |||
+ | ===Step 2 - Alignment and Exposure=== | ||
+ | [[Image:HKU-HKBU_motor_production_2.png | center]] | ||
+ | |||
+ | Note: | ||
+ | # For simple contact, proximity, and projection systems, the mask is the same size and scale as the printed wafer pattern. | ||
+ | # Projection systems give the ability to change the reproduction ratio. Going to 10:1 reduction allows larger size patterns on the mask, which is more robust to mask defects. | ||
+ | # Normally requires at least two alignment mark sets on opposite sides of wafer or stepped region. | ||
+ | # We use "deep ultraviolet", which is produced by excimer lasers, as light source. | ||
+ | |||
+ | ===Step 3 - Dry Etch=== | ||
+ | [[Image:HKU-HKBU_motor_production_3.png | center]] | ||
+ | |||
+ | Note: | ||
+ | # Dry Etching is an etching process that does not utilize any liquid chemicals or etchants to remove materials from the wafer, generating only volatile byproducts in the process. | ||
+ | # Dry etching may be accomplished by any of the following: 1) through chemical reactions that consume the material, using chemically reactive gases or plasma; 2) physical removal of the material, usually by momentum transfer; or 3) a combination of both physical removal and chemical reactions. | ||
+ | # In this project, we use chemically reactive gases to consume Si. | ||
+ | |||
+ | |||
+ | ==Membrane Production== | ||
+ | |||
+ | ===Step 1=== | ||
The Immobolin-P membrane was first made wet and consequently homogenized with a mini-homogenizer. | The Immobolin-P membrane was first made wet and consequently homogenized with a mini-homogenizer. | ||
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[[Image:HKU-HKBU_motor_results_1.png | center]] | [[Image:HKU-HKBU_motor_results_1.png | center]] | ||
- | ==Step 2== | + | ===Step 2=== |
The Immobolin-P membrane was first moistened, then it was put into a 10ml centrifugation tube. The tube was then totally filled with glass beads, and undergo vortexing subsequently. | The Immobolin-P membrane was first moistened, then it was put into a 10ml centrifugation tube. The tube was then totally filled with glass beads, and undergo vortexing subsequently. | ||
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[[Image:HKU-HKBU_motor_results_3.png | center]] | [[Image:HKU-HKBU_motor_results_3.png | center]] | ||
- | ==Step 3== | + | ===Step 3=== |
The Immobolin-P membrane was moistened and liquefied nitrogen was poured onto it. The membrane was broken into pieces by human hands. | The Immobolin-P membrane was moistened and liquefied nitrogen was poured onto it. The membrane was broken into pieces by human hands. | ||
Results: The membrane remained intact. | Results: The membrane remained intact. | ||
- | ==Step 4== | + | ===Step 4=== |
The Immobolin-P membrane was first biotinylated and cut into very small pieces by human hands. Then, the membrane fragments were put into a mould made with aluminium foil and fixed into it with the help of glue. The mould along with the membrane fragment were cut with a Leica-crytomicrotome into further smaller pieces the size of 100umx60umx100um. | The Immobolin-P membrane was first biotinylated and cut into very small pieces by human hands. Then, the membrane fragments were put into a mould made with aluminium foil and fixed into it with the help of glue. The mould along with the membrane fragment were cut with a Leica-crytomicrotome into further smaller pieces the size of 100umx60umx100um. | ||
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[[Image:HKU-HKBU_motor_results_6.png | center]] | [[Image:HKU-HKBU_motor_results_6.png | center]] | ||
- | ==Step 5== | + | ===Step 5=== |
Some elemental silicon fragments were put inside a 1-ml eppendorf tube. The protein-biotin complex and some concentrated HCL were then added into the same tube. The tube was put inside a water bath for 2 hours. The silicon fragments were made dry by rinsing with PBS and followed by air drying. Then, some streptavidin containing beads were added onto the fragments. The fragments were observed under a microscope. | Some elemental silicon fragments were put inside a 1-ml eppendorf tube. The protein-biotin complex and some concentrated HCL were then added into the same tube. The tube was put inside a water bath for 2 hours. The silicon fragments were made dry by rinsing with PBS and followed by air drying. Then, some streptavidin containing beads were added onto the fragments. The fragments were observed under a microscope. | ||
Results: No beads were bound to the silicon fragments. | Results: No beads were bound to the silicon fragments. | ||
- | ==Step 6== | + | ===Step 6=== |
The silicon fragments were silanized by soaking them for 2 h in a solution of aminopropyl triethoxyl silane (3% aminopropyl triethoxyl silane, 2% acetic acid, 5% water, 90% ethanol), then rinsed with ethanol, and dried with a PCR machine for 5 mins. The amino-coated rotors (Fig. 2Bh) were then reacted with 1 mM succinimidyl-6-(biotinamido)-6-hexana- mido hexanoate (EZ-Link NHS-LC-LC-biotin; Pierce, Rock- ford, IL) dissolved in 40 mM phosphate buffer (pH 8.0) for 1 h at 37°C. Then strepatavidin beads were allowed to bind onto it and the fragments were observed under a microscope. | The silicon fragments were silanized by soaking them for 2 h in a solution of aminopropyl triethoxyl silane (3% aminopropyl triethoxyl silane, 2% acetic acid, 5% water, 90% ethanol), then rinsed with ethanol, and dried with a PCR machine for 5 mins. The amino-coated rotors (Fig. 2Bh) were then reacted with 1 mM succinimidyl-6-(biotinamido)-6-hexana- mido hexanoate (EZ-Link NHS-LC-LC-biotin; Pierce, Rock- ford, IL) dissolved in 40 mM phosphate buffer (pH 8.0) for 1 h at 37°C. Then strepatavidin beads were allowed to bind onto it and the fragments were observed under a microscope. | ||
{{Team:HKU-HKBU/footer}} | {{Team:HKU-HKBU/footer}} |
Revision as of 05:41, 14 October 2009
Contents |
Motor - Results
Motor Production
Step 1 - Photoresist (SU-8) Spin Coating
Note:
- Typical contaminants that must be removed prior to photoresist (SU-8) coating.
- Adhesion promoters are used to assist resist coating.
- Ideally want no H2O on wafer surface.
- Wafer is held on a spinner chuck by vacuum and resist is coated to uniform thickness by spin coating.
- Resist thickness is 1-2 mm.
Step 2 - Alignment and Exposure
Note:
- For simple contact, proximity, and projection systems, the mask is the same size and scale as the printed wafer pattern.
- Projection systems give the ability to change the reproduction ratio. Going to 10:1 reduction allows larger size patterns on the mask, which is more robust to mask defects.
- Normally requires at least two alignment mark sets on opposite sides of wafer or stepped region.
- We use "deep ultraviolet", which is produced by excimer lasers, as light source.
Step 3 - Dry Etch
Note:
- Dry Etching is an etching process that does not utilize any liquid chemicals or etchants to remove materials from the wafer, generating only volatile byproducts in the process.
- Dry etching may be accomplished by any of the following: 1) through chemical reactions that consume the material, using chemically reactive gases or plasma; 2) physical removal of the material, usually by momentum transfer; or 3) a combination of both physical removal and chemical reactions.
- In this project, we use chemically reactive gases to consume Si.
Membrane Production
Step 1
The Immobolin-P membrane was first made wet and consequently homogenized with a mini-homogenizer.
Results: The membrane could not be broken into small pieces.
Step 2
The Immobolin-P membrane was first moistened, then it was put into a 10ml centrifugation tube. The tube was then totally filled with glass beads, and undergo vortexing subsequently.
Results: The membrane remained intact.
Step 3
The Immobolin-P membrane was moistened and liquefied nitrogen was poured onto it. The membrane was broken into pieces by human hands.
Results: The membrane remained intact.
Step 4
The Immobolin-P membrane was first biotinylated and cut into very small pieces by human hands. Then, the membrane fragments were put into a mould made with aluminium foil and fixed into it with the help of glue. The mould along with the membrane fragment were cut with a Leica-crytomicrotome into further smaller pieces the size of 100umx60umx100um.
Step 5
Some elemental silicon fragments were put inside a 1-ml eppendorf tube. The protein-biotin complex and some concentrated HCL were then added into the same tube. The tube was put inside a water bath for 2 hours. The silicon fragments were made dry by rinsing with PBS and followed by air drying. Then, some streptavidin containing beads were added onto the fragments. The fragments were observed under a microscope.
Results: No beads were bound to the silicon fragments.
Step 6
The silicon fragments were silanized by soaking them for 2 h in a solution of aminopropyl triethoxyl silane (3% aminopropyl triethoxyl silane, 2% acetic acid, 5% water, 90% ethanol), then rinsed with ethanol, and dried with a PCR machine for 5 mins. The amino-coated rotors (Fig. 2Bh) were then reacted with 1 mM succinimidyl-6-(biotinamido)-6-hexana- mido hexanoate (EZ-Link NHS-LC-LC-biotin; Pierce, Rock- ford, IL) dissolved in 40 mM phosphate buffer (pH 8.0) for 1 h at 37°C. Then strepatavidin beads were allowed to bind onto it and the fragments were observed under a microscope.