Team:HKU-HKBU/motor results
From 2009.igem.org
Contents |
Motor - Results
Motor Production
Step 1 - Photoresist (SU-8) Spin Coating
Note:
- Typical contaminants that must be removed prior to photoresist (SU-8) coating.
- Adhesion promoters are used to assist resist coating.
- Ideally want no H2O on wafer surface.
- Wafer is held on a spinner chuck by vacuum and resist is coated to uniform thickness by spin coating.
- Resist thickness is 1-2 mm.
Step 2 - Alignment and Exposure
Note:
- For simple contact, proximity, and projection systems, the mask is the same size and scale as the printed wafer pattern.
- Projection systems give the ability to change the reproduction ratio. Going to 10:1 reduction allows larger size patterns on the mask, which is more robust to mask defects.
- Normally requires at least two alignment mark sets on opposite sides of wafer or stepped region.
- We use "deep ultraviolet", which is produced by excimer lasers, as light source.
Step 3 - Dry Etch
Note:
- Dry Etching is an etching process that does not utilize any liquid chemicals or etchants to remove materials from the wafer, generating only volatile byproducts in the process.
- Dry etching may be accomplished by any of the following: 1) through chemical reactions that consume the material, using chemically reactive gases or plasma; 2) physical removal of the material, usually by momentum transfer; or 3) a combination of both physical removal and chemical reactions.
- In this project, we use chemically reactive gases to consume Si.
Step 4 - Photoresist (SU-8) Spin Coating
Note:
Please refer to Step 1.
Membrane Production
Step 1
The Immobolin-P membrane was first made wet and consequently homogenized with a mini-homogenizer.
Results: The membrane could not be broken into small pieces.
Step 2
The Immobolin-P membrane was first moistened, then it was put into a 10ml centrifugation tube. The tube was then totally filled with glass beads, and undergo vortexing subsequently.
Results: The membrane remained intact.
Step 3
The Immobolin-P membrane was moistened and liquefied nitrogen was poured onto it. The membrane was broken into pieces by human hands.
Results: The membrane remained intact.
Step 4
The Immobolin-P membrane was first biotinylated and cut into very small pieces by human hands. Then, the membrane fragments were put into a mould made with aluminium foil and fixed into it with the help of glue. The mould along with the membrane fragment were cut with a Leica-crytomicrotome into further smaller pieces the size of 100umx60umx100um.
Step 5
Some elemental silicon fragments were put inside a 1-ml eppendorf tube. The protein-biotin complex and some concentrated HCL were then added into the same tube. The tube was put inside a water bath for 2 hours. The silicon fragments were made dry by rinsing with PBS and followed by air drying. Then, some streptavidin containing beads were added onto the fragments. The fragments were observed under a microscope.
Results: No beads were bound to the silicon fragments.
Step 6
The silicon fragments were silanized by soaking them for 2 h in a solution of aminopropyl triethoxyl silane (3% aminopropyl triethoxyl silane, 2% acetic acid, 5% water, 90% ethanol), then rinsed with ethanol, and dried with a PCR machine for 5 mins. The amino-coated rotors (Fig. 2Bh) were then reacted with 1 mM succinimidyl-6-(biotinamido)-6-hexana- mido hexanoate (EZ-Link NHS-LC-LC-biotin; Pierce, Rock- ford, IL) dissolved in 40 mM phosphate buffer (pH 8.0) for 1 h at 37°C. Then strepatavidin beads were allowed to bind onto it and the fragments were observed under a microscope.