"
Team:HKU-HKBU/motor results
From 2009.igem.org
Contents |
Motor - Results
Step 1
The Immobolin-P membrane was first made wet and consequently homogenized with a mini-homogenizer.
Results: The membrane could not be broken into small pieces.
Step 2
The Immobolin-P membrane was first moistened, then it was put into a 10ml centrifugation tube. The tube was then totally filled with glass beads, and undergo vortexing subsequently.
Results: The membrane remained intact.
Step 3
The Immobolin-P membrane was moistened and liquefied nitrogen was poured onto it. The membrane was broken into pieces by human hands.
Results: The membrane remained intact.
Step 4
The Immobolin-P membrane was first biotinylated and cut into very small pieces by human hands. Then, the membrane fragments were put into a mould made with aluminium foil and fixed into it with the help of glue. The mould along with the membrane fragment were cut with a Leica-crytomicrotome into further smaller pieces the size of 100umx60umx100um.
