Team:HKUST/Back4

From 2009.igem.org

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The binary toxin BinA and BinB, which is produced in ''Bacillus sphaericus'', is a mosquito-larvicidal crystal protein. It has its maximum activity when both components are present in equimolar ratio[1]. It could kill larvae by forming pores once binA binds to and binB inserts to membrane lipid bilayer, leading to swelling and lysis of the cell[2]. </p>
The binary toxin BinA and BinB, which is produced in ''Bacillus sphaericus'', is a mosquito-larvicidal crystal protein. It has its maximum activity when both components are present in equimolar ratio[1]. It could kill larvae by forming pores once binA binds to and binB inserts to membrane lipid bilayer, leading to swelling and lysis of the cell[2]. </p>
Attempts have been made to produce the binary toxin at a high level in many organisms including ''Escherichia coli''[3]. To obtain a high expression level and a high-potency toxin, one approach is to improve solubility by tagging T7 tag [4] or glutathione S-transferase (GST), between which GST seems to work better. Not only does GST increase the protein expression level, it also improves the solubility of these proteins as well[2].</p>
Attempts have been made to produce the binary toxin at a high level in many organisms including ''Escherichia coli''[3]. To obtain a high expression level and a high-potency toxin, one approach is to improve solubility by tagging T7 tag [4] or glutathione S-transferase (GST), between which GST seems to work better. Not only does GST increase the protein expression level, it also improves the solubility of these proteins as well[2].</p>
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Yeast has also been a good tool for bacterial toxin studies. It can functionally express many bacterial toxins[5]. Since the binary toxin Bin A and Bin B are very specific to insects, we propose that yeast can express this toxin without damaging itself; at the same time, when insects eat the yeast the insect will die. This is why we choose this binary toxin as our “insect killer”.<br>
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Yeast has also been a good tool for bacterial toxin studies. It can functionally express many bacterial toxins[5]. Since the binary toxin Bin A and Bin B are very specific to insects, we propose that yeast can express this toxin without damaging itself; at the same time, insects will be killed by the toxin if they eat the yeast. This is why we choose this binary toxin as our “insect killer”.<br>
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<li><a href="https://2009.igem.org/Team:HKUST/Back4">Background</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/Back4">Background</a></li>

Revision as of 10:02, 20 October 2009

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The binary toxin BinA and BinB, which is produced in ''Bacillus sphaericus'', is a mosquito-larvicidal crystal protein. It has its maximum activity when both components are present in equimolar ratio[1]. It could kill larvae by forming pores once binA binds to and binB inserts to membrane lipid bilayer, leading to swelling and lysis of the cell[2].

Attempts have been made to produce the binary toxin at a high level in many organisms including ''Escherichia coli''[3]. To obtain a high expression level and a high-potency toxin, one approach is to improve solubility by tagging T7 tag [4] or glutathione S-transferase (GST), between which GST seems to work better. Not only does GST increase the protein expression level, it also improves the solubility of these proteins as well[2].

Yeast has also been a good tool for bacterial toxin studies. It can functionally express many bacterial toxins[5]. Since the binary toxin Bin A and Bin B are very specific to insects, we propose that yeast can express this toxin without damaging itself; at the same time, insects will be killed by the toxin if they eat the yeast. This is why we choose this binary toxin as our “insect killer”.

  • Background
  • Experimental Design
  • Parts Design
  • Future Work
  • References
  • HKUST