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Team:HKUST/Protocols
From 2009.igem.org
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Western_blotting"> Western blotting</a></li> | <li><a href="https://2009.igem.org/Team:HKUST/Protocols/Western_blotting"> Western blotting</a></li> | ||
<li><a href="https://2009.igem.org/Team:HKUST/Protocols/GFP_testing"> GFP testing</a></li> | <li><a href="https://2009.igem.org/Team:HKUST/Protocols/GFP_testing"> GFP testing</a></li> | ||
| - | + | ||
</ul> | </ul> | ||
Revision as of 15:22, 19 October 2009
a
- Agarose gel preparation and gel electrophoresis
- PCR (Taq, Vent)
- PCR product clean-up
- Enzyme digestion (vector, insert)
- Plasmid DNA extraction
- Gel cutting
- Gel extraction
- Ethanol precipitation
- Ligation
- Transformation to E.coli
- Transformation to yeast
- Cell lysis
- Yeast genomic DNA extraction
- Western blotting
- GFP testing
iGEM 2009
