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Team:HKUST/Protocols/GFP testing
From 2009.igem.org
Fluorescence microscopy visualization
- 1. Grow yeast transformed cells containing appropriate plasmids in 5 ml SCM minus His & glucose at 30ºC overnight..
- 2. Add galactose (to a conc.of 2%) at a cell density corresponding to an OD600 of a 10-fold dilution reaching 0.8.
- 3. Continue growing cells at 30ºC for 30min to 1.5hr.
- 4. At time 30min, 60min, 90min, use 1ml of cells, spin down and resuspend cells in 10μl ddH2O.
- 5. Use 2μl cells and visualize the cells in a fluorescency microscopy.
