Team:HKUST/Protocols/gel cut

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6. Cutting bands on the gel 

  Purpose: To extract specific DNA fragment.
  Procedure: 
  a.Visualize the bands under UV light. Use long-wavelength UV to minimize damage to the DNA.
  b.Cut the band with a clean razor blade.
  c.Turn the gel slice on its side to trim off extra agarose. Place the gel in a microcentrifuge tube.
  Tips:
  •EB staining is needed for higher resolution under UV light.
  Safety Tips: 
  •Permission is needed from technician before doing this! Follow the safety instruction in the room.
  •When using UV light, protect your skin by wearing safety goggles or a face shield, gloves, and a lab coat.
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