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Team:HKUST/Protocols/gel extraction
From 2009.igem.org
7. Gel extraction
Purpose: To clean up the gel after band cutting from the gel and leave only the desired DNA fragment. Materials: GEX Buffer, WN Buffer, WS Buffer, Elution Buffer (all provided in FavorPrepTM Gel/PCR DNA Clean-Up Kit) Procedure: a.Add 0.5ml GEX buffer into the tube with gel fragment in it. b.Incubate at 60°C for 5-10mins until the gel is completely dissolved. Invert the tube every 2 mins during the incubation. Stop incubation after the gel is completely dissolved. Let the gel mixture to cool down to room temperature. c.Place a GP column onto a collection tube. Load no more than 0.7ml gel mixture into each column. Centrifuge for 30s-60s (9000rpm). Discard the flow through. d.Repeat step c for the excessive gel mixture. e.Wash the column once with 0.5ml WN buffer by centrifuging for 30s-60s (9000rpm). Discard the flow through. f.Wash the column once with 0.5ml WS buffer by centrifuging for 30s-60s (9000rpm).
