"
Team:HKUST/Ref1
From 2009.igem.org
(Difference between revisions)
| (12 intermediate revisions not shown) | |||
| Line 18: | Line 18: | ||
<li><a href="https://2009.igem.org/Team:HKUST">Home</a></li> | <li><a href="https://2009.igem.org/Team:HKUST">Home</a></li> | ||
<li><a href="https://2009.igem.org/Team:HKUST/Team">Our Team</a></li> | <li><a href="https://2009.igem.org/Team:HKUST/Team">Our Team</a></li> | ||
| - | <li><a href="https://2009.igem.org/Team:HKUST/Project">Project | + | <li><a href="https://2009.igem.org/Team:HKUST/Project">Project Description</a></li> |
| - | < | + | <b> |
| - | < | + | <span style="color:green"> |
| - | <li> | + | <li>Main Parts</li> |
| - | + | </span> | |
| - | + | </b> | |
| - | < | + | <li><a href="https://2009.igem.org/Team:HKUST/OdorantSensing">Odorant Sensing</a></li> |
| - | < | + | <li><a href="https://2009.igem.org/Team:HKUST/AttractantProduction">Attractant Production</a></li> |
| - | + | <li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | <li><a href="https://2009.igem.org/Team:HKUST/ | + | |
| - | <li><a href="https://2009.igem.org/Team:HKUST/ | + | |
| - | <li><a href="https://2009.igem.org/Team:HKUST/ | + | |
| - | < | + | <b> |
| - | < | + | <span style="color:green"> |
| - | <li> | + | <li>Resources</li> |
| - | < | + | </span> |
| - | < | + | </b> |
| - | + | ||
| - | + | ||
| - | + | ||
<li><a href="https://2009.igem.org/Team:HKUST/Lab Notebook">Lab Notebook</a></li> | <li><a href="https://2009.igem.org/Team:HKUST/Lab Notebook">Lab Notebook</a></li> | ||
<li><a href="https://2009.igem.org/Team:HKUST/Parts">Parts Submitted </a></li> | <li><a href="https://2009.igem.org/Team:HKUST/Parts">Parts Submitted </a></li> | ||
| - | <li><a href="https://2009.igem.org/Team:HKUST/Protocols">Protocol | + | <li><a href="https://2009.igem.org/Team:HKUST/Protocols">Protocol List</a></li> |
| - | <li><a href="https://2009.igem.org/Team:HKUST/Resourses">Other | + | <li><a href="https://2009.igem.org/Team:HKUST/Resourses">Other Resources</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 56: | Line 44: | ||
<ul> | <ul> | ||
<li><a href="https://2009.igem.org/Team:Gallery">Gallery</a></li> | <li><a href="https://2009.igem.org/Team:Gallery">Gallery</a></li> | ||
| - | <li><a href="https://2009.igem.org/Team: | + | <li><a href="https://2009.igem.org/Team:Biosafety">Biosafety</a></li> |
<li><a href="https://2009.igem.org/Team:Acknowledgement">Acknowledgement</a></li> | <li><a href="https://2009.igem.org/Team:Acknowledgement">Acknowledgement</a></li> | ||
</ul> | </ul> | ||
| Line 62: | Line 50: | ||
</div> | </div> | ||
<div id="rightxx"> | <div id="rightxx"> | ||
| + | <div class="contentodS_r"> <h3>a</h3> | ||
| + | </div> | ||
<div class="contentxx"> | <div class="contentxx"> | ||
| - | + | </p> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| + | [1] Bryson-Richardson RJ, Logan DW, Currie PD & Jackson IJ. Large-scale analysis of gene structure in rhodopsin-like GPCRs: evidence for widespread loss of an ancient intron. <em>Gene</em> <b>338</b> , 15–23 (2004). <br><br> | ||
| + | [2] Jacoby E, Bouhelal R, Gerspacher M & Seuwen K. The 7 TM G-protein-coupled receptor target family. <em>ChemMedChem</em> <b>1</b> , 761–782 (2006).<br><br> | ||
| + | [3] Suga H & Haga T. Ligand screening system using fusion proteins of G protein-coupled receptors with G protein α subunits. <em>Neurochemistry International</em> <b>51</b>, 140-164 (2007).<br><br> | ||
| + | [4] Sato K & Touhara K. Insect olfaction: receptors, signal transduction, and behavior. <em>Results Probl. Cell Differ. Epub</em>, ahead of print (2008).<br><br> | ||
| + | [5] Versele M, <em>et al</em>. Sex and sugar in yeast: two distinct GPCR | ||
| + | systems. <em>EMBO Rep.</em> <b> 2</b>, 574–579 (2001).<br><br> | ||
| + | [6] Ladds G, Goddard A & Davey J. Functional analysis of heterologous GPCR signalling pathways in yeast.<em>Trends in Biotechnology</em><b> 23</b>, 367-373 (2005).<br><br> | ||
| + | [7] Johnson GL & Dhanasekaran N. The G-protein family and their interaction with receptors. <em>Endocr Rev. </em> <b>10</b>, 317–331 (1989).<br><br> | ||
| + | [8] Minic J, Persuy MA, Godel E, Aioun J, Connerton I, Salesse R & Pajot-Augy E. Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening. <em>FEBS J.</em> <b>272</b>, 524–537 (2005).<br><br> | ||
| + | [9] Minic J, Sautel M, Salesse R & Pajot-Augy E. Yeast System as a Screening Tool for Pharmacological Assessment of G Protein Coupled Receptors. <em>Curr. Med. Chem.</em> <b>12</b>, 961-969 (2005).<br><br> | ||
| + | [10] Radhika V, Proikas-Cezanne T, Jayaraman M, Onesime D, Ha JH, Dhanasekaran DN. Chemical sensing of DNT by engineered olfactory yeast strain. <em>Nat. Chem. Biol.</em> <b>3</b>, 325–330 (2007)<br><br> | ||
| + | [11] Crowe ML, Perry BN & Connerton IF. Golf complements a GPA1 null mutation in Saccharomyces cerevisiae and functionally couples to the STE2 pheromone receptor. <em>J Recept Signal Transduct Res </em> <b>20</b>, 61–73 (2000). <br><br> | ||
| + | [12] Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H <em>et al</em>. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. <em>Yeast</em> <b>21</b> , 947–962 (2004).<br><br> | ||
| + | [13] Zhang Y, Chou JH, Bradley J, Bargmann CI & Zinn K. The Caenorhabditis elegans seven-transmembrane protein ODR-10 functions as an odorant receptor in mammalian cells. <em>Proc. Natl. Acad. Sci.</em> <b>94</b>, 12162–12167 (1997).<br> | ||
| + | <br> | ||
| + | <li><a href="https://2009.igem.org/Team:HKUST/Back1">Background</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:HKUST/Group1">Experimental Design</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:HKUST/Part1">Parts Design</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:HKUST/Result1">Experimental Results</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:HKUST/Future1">Future Work</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:HKUST/Ref1">References</a></li> | ||
| Line 100: | Line 94: | ||
<span> iGEM 2009 <br /> </span> | <span> iGEM 2009 <br /> </span> | ||
</div> | </div> | ||
| - | <div id="payment"><img src="http://igem2009hkust.fileave.com/wiki/template/12092009/images/HKUSTLogo.jpg" alt=" | + | <div id="payment"><img src="http://igem2009hkust.fileave.com/wiki/template/12092009/images/HKUSTLogo.jpg" alt="HKUST" /></div> |
</div> | </div> | ||
<div id="footerend"></div> | <div id="footerend"></div> | ||
Latest revision as of 13:46, 20 October 2009
a
[1] Bryson-Richardson RJ, Logan DW, Currie PD & Jackson IJ. Large-scale analysis of gene structure in rhodopsin-like GPCRs: evidence for widespread loss of an ancient intron. Gene 338 , 15–23 (2004).
[2] Jacoby E, Bouhelal R, Gerspacher M & Seuwen K. The 7 TM G-protein-coupled receptor target family. ChemMedChem 1 , 761–782 (2006).
[3] Suga H & Haga T. Ligand screening system using fusion proteins of G protein-coupled receptors with G protein α subunits. Neurochemistry International 51, 140-164 (2007).
[4] Sato K & Touhara K. Insect olfaction: receptors, signal transduction, and behavior. Results Probl. Cell Differ. Epub, ahead of print (2008).
[5] Versele M, et al. Sex and sugar in yeast: two distinct GPCR systems. EMBO Rep. 2, 574–579 (2001).
[6] Ladds G, Goddard A & Davey J. Functional analysis of heterologous GPCR signalling pathways in yeast.Trends in Biotechnology 23, 367-373 (2005).
[7] Johnson GL & Dhanasekaran N. The G-protein family and their interaction with receptors. Endocr Rev. 10, 317–331 (1989).
[8] Minic J, Persuy MA, Godel E, Aioun J, Connerton I, Salesse R & Pajot-Augy E. Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening. FEBS J. 272, 524–537 (2005).
[9] Minic J, Sautel M, Salesse R & Pajot-Augy E. Yeast System as a Screening Tool for Pharmacological Assessment of G Protein Coupled Receptors. Curr. Med. Chem. 12, 961-969 (2005).
[10] Radhika V, Proikas-Cezanne T, Jayaraman M, Onesime D, Ha JH, Dhanasekaran DN. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3, 325–330 (2007)
[11] Crowe ML, Perry BN & Connerton IF. Golf complements a GPA1 null mutation in Saccharomyces cerevisiae and functionally couples to the STE2 pheromone receptor. J Recept Signal Transduct Res 20, 61–73 (2000).
[12] Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H et al. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast 21 , 947–962 (2004).
[13] Zhang Y, Chou JH, Bradley J, Bargmann CI & Zinn K. The Caenorhabditis elegans seven-transmembrane protein ODR-10 functions as an odorant receptor in mammalian cells. Proc. Natl. Acad. Sci. 94, 12162–12167 (1997).
Background
Experimental Design
Parts Design
Experimental Results
Future Work
References
[2] Jacoby E, Bouhelal R, Gerspacher M & Seuwen K. The 7 TM G-protein-coupled receptor target family. ChemMedChem 1 , 761–782 (2006).
[3] Suga H & Haga T. Ligand screening system using fusion proteins of G protein-coupled receptors with G protein α subunits. Neurochemistry International 51, 140-164 (2007).
[4] Sato K & Touhara K. Insect olfaction: receptors, signal transduction, and behavior. Results Probl. Cell Differ. Epub, ahead of print (2008).
[5] Versele M, et al. Sex and sugar in yeast: two distinct GPCR systems. EMBO Rep. 2, 574–579 (2001).
[6] Ladds G, Goddard A & Davey J. Functional analysis of heterologous GPCR signalling pathways in yeast.Trends in Biotechnology 23, 367-373 (2005).
[7] Johnson GL & Dhanasekaran N. The G-protein family and their interaction with receptors. Endocr Rev. 10, 317–331 (1989).
[8] Minic J, Persuy MA, Godel E, Aioun J, Connerton I, Salesse R & Pajot-Augy E. Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening. FEBS J. 272, 524–537 (2005).
[9] Minic J, Sautel M, Salesse R & Pajot-Augy E. Yeast System as a Screening Tool for Pharmacological Assessment of G Protein Coupled Receptors. Curr. Med. Chem. 12, 961-969 (2005).
[10] Radhika V, Proikas-Cezanne T, Jayaraman M, Onesime D, Ha JH, Dhanasekaran DN. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3, 325–330 (2007)
[11] Crowe ML, Perry BN & Connerton IF. Golf complements a GPA1 null mutation in Saccharomyces cerevisiae and functionally couples to the STE2 pheromone receptor. J Recept Signal Transduct Res 20, 61–73 (2000).
[12] Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H et al. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast 21 , 947–962 (2004).
[13] Zhang Y, Chou JH, Bradley J, Bargmann CI & Zinn K. The Caenorhabditis elegans seven-transmembrane protein ODR-10 functions as an odorant receptor in mammalian cells. Proc. Natl. Acad. Sci. 94, 12162–12167 (1997).
iGEM 2009
