Team:HKUST/Ref1

From 2009.igem.org

Salt and Soap template

a

[1] Bryson-Richardson RJ, Logan DW, Currie PD & Jackson IJ. Large-scale analysis of gene structure in rhodopsin-like GPCRs: evidence for widespread loss of an ancient intron. Gene 338 , 15–23 (2004).

[2] Jacoby E, Bouhelal R, Gerspacher M & Seuwen K. The 7 TM G-protein-coupled receptor target family. ChemMedChem 1 , 761–782 (2006).

[3] Suga H & Haga T. Ligand screening system using fusion proteins of G protein-coupled receptors with G protein α subunits. Neurochemistry International 51, 140-164 (2007).

[4] Sato K & Touhara K. Insect olfaction: receptors, signal transduction, and behavior. Results Probl. Cell Differ. Epub, ahead of print (2008).

[5] Versele M, et al. Sex and sugar in yeast: two distinct GPCR systems. EMBO Rep. 2, 574–579 (2001).

[6] Ladds G, Goddard A & Davey J. Functional analysis of heterologous GPCR signalling pathways in yeast.Trends in Biotechnology 23, 367-373 (2005).

[7] Johnson GL & Dhanasekaran N. The G-protein family and their interaction with receptors. Endocr Rev. 10, 317–331 (1989).

[8] Minic J, Persuy MA, Godel E, Aioun J, Connerton I, Salesse R & Pajot-Augy E. Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening. FEBS J. 272, 524–537 (2005).

[9] Minic J, Sautel M, Salesse R & Pajot-Augy E. Yeast System as a Screening Tool for Pharmacological Assessment of G Protein Coupled Receptors. Curr. Med. Chem. 12, 961-969 (2005).

[10] Radhika V, Proikas-Cezanne T, Jayaraman M, Onesime D, Ha JH, Dhanasekaran DN. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3, 325–330 (2007)

[11] Crowe ML, Perry BN & Connerton IF. Golf complements a GPA1 null mutation in Saccharomyces cerevisiae and functionally couples to the STE2 pheromone receptor. J Recept Signal Transduct Res 20, 61–73 (2000).

[12] Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H et al. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast 21 , 947–962 (2004).

[13] Zhang Y, Chou JH, Bradley J, Bargmann CI & Zinn K. The Caenorhabditis elegans seven-transmembrane protein ODR-10 functions as an odorant receptor in mammalian cells. Proc. Natl. Acad. Sci. 94, 12162–12167 (1997).

  • Background
  • Experimental Design
  • Parts Design
  • Experimental Results
  • Future Work
  • References
  • HKUST