Team:Harvard/Parts

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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your teams namespace. 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:Harvard | Team Example]]
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!align="center"|[[Team:Harvard|Home]]
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!align="center"|[[Team:Harvard/Team|The Team]]
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!align="center"|[[Team:Harvard/Project|The Project]]
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!align="center"|[[Team:Harvard/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Harvard/Modeling|Modeling]]
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!align="center"|[[Team:Harvard/Notebook|Notebook]]
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===Note===
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#content
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
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<h2><a href="https://2009.igem.org/Team:Harvard"><img src="https://static.igem.org/mediawiki/2009/b/b8/Harvard2009Logo.jpg" alt="Hi Mom" width="900" height="200"></a></h2>
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<div style="width: 650px; height: 800px; left: 10px;">
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<table width="650px" border="0" cellspacing="10" cellpadding="0">
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  <tr>
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    <td>
 +
<h2> Parts </h2>
 +
/***********************************************************************************************************************/
 +
        <p>
 +
 
 +
<b>
 +
<br>
 +
Biobrick part#1
 +
<br>
 +
Bba_K207000
 +
<br>
 +
PhyB N-terminal fragment sequence
 +
<br>
 +
Registry vector:
 +
<br>
 +
Plasmid:  pSP3K3 ,Kan resistance
 +
<br>
 +
</b>
 +
Sequence:
 +
 
 +
atggtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggag
 +
 
 +
gagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagact
 +
 
 +
ccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacag
 +
 
 +
atcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttaca
 +
 
 +
gtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgtt
 +
 
 +
cacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccg
 +
 
 +
ttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaat
 +
 
 +
cgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggactt
 +
 
 +
gactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattgga
 +
 
 +
ctgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttg
 +
 
 +
tggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatc
 +
 
 +
tattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggtt
 +
 
 +
gtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgc
 +
 
 +
agttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagag
 +
 
 +
tcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaa
 +
 
 +
gatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttagggg
 +
 
 +
atgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaa
 +
 
 +
gcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaa
 +
 
 +
actgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatct
 +
   
 +
 +
<br><br><br><br><br><br><br>
 +
 +
<b>
 +
Biobrick part#2
 +
<br>
 +
Bba_K207001
 +
<br>
 +
PhyB N-terminal fragment-DBD fusion sequence
 +
<br>
 +
(GAL4 Binding Domain of pGBKT7, highlighted in bold)
 +
 +
<br>
 +
Registry vector:
 +
<br>
 +
Plasmid: pSB12A, Amp resistance
 +
<br>
 +
Subcloned from part # E0040
 +
<br>
 +
Biobrick part insert sequence:
 +
 +
<br>
 +
</b>
 +
Sequence:
 +
 
 +
atggtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggag
 +
 
 +
gagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagact
 +
 
 +
ccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacag
 +
 
 +
atcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttaca
 +
 
 +
gtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgtt
 +
 
 +
cacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccg
 +
 
 +
ttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaat
 +
 
 +
cgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggactt
 +
 
 +
gactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattgga
 +
 
 +
ctgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttg
 +
 
 +
tggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatc
 +
 
 +
tattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggtt
 +
 
 +
gtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgc
 +
 
 +
agttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagag
 +
 
 +
tcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaa
 +
 
 +
gatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttagggg
 +
 
 +
atgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaa
 +
 
 +
gcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaa
 +
 
 +
actgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatct ATGAAGCTAC TGTCTTCTAT CGAACAAGCATGCGATATTT GCCGACTTAA AAAGCTCAAG TGCTCCAAAG AAAAACCGAA GTGCGCCAAG TGTCTGAAGA ACAACTGGGA GTGTCGCTAC TCTCCCAAAA CCAAAAGGTC TCCGCTGACT AGGGCACATC TGACAGAAGT GGAATCAAGG CTAGAAAGAC TGGAACAGCT ATTTCTACTG  ATTTTTCCTC GAGAAGACCT TGACATGATT TTGAAAATGG ATTCTTTACA GGATATAAAA GCATTGTTAA CAGGATTATT TGTACAAGAT AATGTGAATA AAGATGCCGT CACAGATAGA TTGGCTTCAG TGGAGACTGA TATGCCTCTA ACATTGAGAC AGCATAGAAT AAGTGCGACA
 +
TCATCATCGG AAGAGAGTAG TAACAAAGGT CAAAGACAGT TGACTGTATC
 +
 +
 
 +
 
 +
 
 +
</p>
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
  </tr>
 +
</table>
 +
</div>
 +
 
 +
<div style="position: absolute; left: 630px; top: 240px; height: 800px; width: 300px; padding: 3em;">
 +
<table width="300px" border="0" cellspacing="10" cellpadding="0">
 +
  <tr>
 +
    <td>
 +
<h2><a href="https://2009.igem.org/Team:Harvard/Team">Team</a></h2>
 +
<h2><p>Projects</p></h2>
 +
    <ul>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Comm">Bacteria-to-Yeast Communication</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Laser">Characterization</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/PCB">PCB Biosynthesis in Yeast</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Split">Split Luciferase</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Blackboard">Other Possible Reporters</a></li>
 +
 
 +
  </ul>
 +
<h2><p><a href="https://2009.igem.org/Team:Harvard/Parts">Parts</a></p></h2>
 +
        <h2><p><a href="https://2009.igem.org/Team:Harvard/References">References</a></p></h2>
 +
<h2><p>Lab Notebook</p></h2>
 +
    <ul>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Daily">Daily</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Protocols">Protocols</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Recipes">Recipes </a></li>
 +
    </ul>
 +
<h2><p><a href="https://2009.igem.org/Team:Harvard/Ethics">Ethics</a></h2>
 +
        <h2><p><a href="https://2009.igem.org/Team:Harvard/Safety">Safety</a></h2>
 +
        <h2><p><a href="https://2009.igem.org/Team:Harvard/Acknowledgements">Acknowledgements</a></h2>
 +
</td>
 +
  </tr>
 +
</table>
 +
</div>
 +
 
 +
</body>
 +
</html>

Latest revision as of 03:42, 22 October 2009

Hi Mom

Parts

/***********************************************************************************************************************/


Biobrick part#1
Bba_K207000
PhyB N-terminal fragment sequence
Registry vector:
Plasmid: pSP3K3 ,Kan resistance
Sequence: atggtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggag gagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagact ccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacag atcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttaca gtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgtt cacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccg ttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaat cgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggactt gactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattgga ctgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttg tggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatc tattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggtt gtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgc agttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagag tcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaa gatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttagggg atgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaa gcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaa actgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatct






Biobrick part#2
Bba_K207001
PhyB N-terminal fragment-DBD fusion sequence
(GAL4 Binding Domain of pGBKT7, highlighted in bold)
Registry vector:
Plasmid: pSB12A, Amp resistance
Subcloned from part # E0040
Biobrick part insert sequence:
Sequence: atggtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggag gagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagact ccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacag atcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttaca gtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgtt cacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccg ttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaat cgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggactt gactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattgga ctgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttg tggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatc tattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggtt gtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgc agttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagag tcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaa gatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttagggg atgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaa gcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaa actgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatct ATGAAGCTAC TGTCTTCTAT CGAACAAGCATGCGATATTT GCCGACTTAA AAAGCTCAAG TGCTCCAAAG AAAAACCGAA GTGCGCCAAG TGTCTGAAGA ACAACTGGGA GTGTCGCTAC TCTCCCAAAA CCAAAAGGTC TCCGCTGACT AGGGCACATC TGACAGAAGT GGAATCAAGG CTAGAAAGAC TGGAACAGCT ATTTCTACTG ATTTTTCCTC GAGAAGACCT TGACATGATT TTGAAAATGG ATTCTTTACA GGATATAAAA GCATTGTTAA CAGGATTATT TGTACAAGAT AATGTGAATA AAGATGCCGT CACAGATAGA TTGGCTTCAG TGGAGACTGA TATGCCTCTA ACATTGAGAC AGCATAGAAT AAGTGCGACA TCATCATCGG AAGAGAGTAG TAACAAAGGT CAAAGACAGT TGACTGTATC

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References

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Acknowledgements

Retrieved from "http://2009.igem.org/Team:Harvard/Parts"