Further Subprojects

• We studied, cloned and characterized some natural promoters
• We created a preliminary stable cell line containing a FRT site(s).
• We attempted to create a dual assay plasmid for promoter measurement, which should have the advantage of two fluorescent proteins with different promoters. One of them would be constitutive the other one to be tested. This construction would allow a standardized comparison of promoter strength, due to the elimination of different transfection rates.

Unfortunately the repeated cloning of the construct in Fig 1. was not successful.