Team:Heidelberg/k4l1um

From 2009.igem.org

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'''SH''':  
'''SH''':  
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* [[Team:Heidelberg/n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)|Miniprep]]Miniprep of GFP template plasmid, pcDNA5/FRT
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* [[Team:Heidelberg/n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)|Miniprep]] of GFP template plasmid, pcDNA5/FRT
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* Maxiprep pcDNA/FRT 188.7ng/µL
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* [[Team:Heidelberg/n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)|Maxiprep]] pcDNA/FRT 188.7ng/µL
'''LV''':  
'''LV''':  
* Extraction of CMV promoter from 2008 distribution
* Extraction of CMV promoter from 2008 distribution
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* Transformation of DH5a cell with CMV promoter
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* [[Team:Heidelberg/n4tr1um# Transformation of Bacteria|Transformation]] of DH5a cell with CMV promoter
* portzughtr
* portzughtr
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Lab: LV, SH, CZ
Lab: LV, SH, CZ
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* Annealing of oligos (JeT, cFos, Min)
+
* Annealing of Oligos (JeT, cFos, Min)
* Purification of cDNA via 2% / 3% agarosegel  
* Purification of cDNA via 2% / 3% agarosegel  
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* Site directed mutagenesis of pcDNA5/FRT  
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* [[Team:Heidelberg/n4tr1um#Site-directed mutagenesis|Site directed mutagenesis]] of pcDNA5/FRT  
* Dpn1 digestion
* Dpn1 digestion
* Transformation of DH5a
* Transformation of DH5a

Revision as of 13:41, 17 June 2009

6-15-2009

Lab: LV, SH, CZ

SH:

  • Miniprep of GFP template plasmid, pcDNA5/FRT
Nr pcDNA5/FRT GFP
1 4,7 39,7
2 5,9 14,9
3 3,7 3,8
4 5,0 7,8


LV:

  • Extraction of CMV promoter from 2008 distribution
  • Transformation of DH5a cell with CMV promoter
  • portzughtr

6-16-2009

Lab: LV, SH, CZ

  • Annealing of Oligos (JeT, cFos, Min)
  • Purification of cDNA via 2% / 3% agarosegel
Retrieved from "http://2009.igem.org/Team:Heidelberg/k4l1um"