Team:IBB Pune/Project

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We are a team initiated by students. This is our first year at iGEM and we are excited to be a part of the whole iGEM experience. We are proud to be the first student representatives of IBB at any international level competition.

We are a group of 7 undergrads currently studying biotechnology in the third, fourth and fifth years of the Integrated Master's course at IBB, University Pune. We are a bunch of creative, hardworking and enthusiastic students. The team members know each others strengths and weaknesses very well and that makes us work as a team. We hope to succeed this year in iGEM and make a mark in the field of synthetic biology at the international level.

Contents

Brainstorming

We have had many brainstorming sessions within the team and with the instructors in which hundreds of ideas were discussed. We are listing a few interesting ideas below and we are sure that some or the other team will get rid of the theoretical/practical difficulties we faced and will do projects related to these ideas the next year if not this year.

1.Sudoku solver bacteria: This is quite a popular problem and many teams have considered doing this. We chose to solve a 4 x 4 sudoku because it is much simpler than the usual 9 x 9 one. The sudoku solving bacteria are a strain of bacteria, that will have a genetic circuit that allows differentiation into 4 types of phenotypes corresponding to the numbers 1,2,3,4. These phenotypes will include a combination of reporter proteins and signal molecules that will inhibit the differentiation to that specific phenotype. eg. "1" will inhibit the differentiation of naive cells to the 1 phenotype along rows, columns and blocks and so on. There are however many technical challenges. How do we restrict inhibition to a single box / column / row without disturbing other boxes?

Overall project

1. Export tag synthesis and inducible export of desired proteins for in vivo simulation of the SNOWDRIFT game.

2. Bacterial Turing machines. We are trying to make small two-state Turing machines like unary adder and attempting to build an AND gate.

3. inducible natural competence and lysis using genes from naturally competent bacteria and phages.

Project Details

Part 2

The Experiments

Part 3

== Results ==