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Team:IBB Pune/protein-based-signalling
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(→Working of the Construct) |
(→Working of the Construct) |
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In the above construct, there are three independent modules that function | In the above construct, there are three independent modules that function | ||
| - | * A constitutively working module which produces LuxR protein and LacI | + | * A constitutively working module which produces LuxR protein and LacI and Cassette 1 (encodes ogr and activates state 'B') |
| - | *A State-'B' | + | *A State-'B' which is activated by State' A' and which in turn represses the working of the default state |
*An AND gate regulated activation of the turing machine function (it could be lysis, expression of a fluorescent protein or the synthesis of vanillin or methyl salicylate) | *An AND gate regulated activation of the turing machine function (it could be lysis, expression of a fluorescent protein or the synthesis of vanillin or methyl salicylate) | ||
The default state of the turing machine is 'A'. | The default state of the turing machine is 'A'. | ||
In this state it encounters a '0' (represented by Lactose) first.However there are no Lactose responsive elements in either the first or the second module. | In this state it encounters a '0' (represented by Lactose) first.However there are no Lactose responsive elements in either the first or the second module. | ||
| - | The third Module which has such a module however also requires the presence of | + | The third Module which has such a module however also requires the presence of the phage protein 'ogr ' |
| + | . Hence the first 0 is unable to produce any effect and the machine moves on to the next cell in the tape while remaining in state A. | ||
| + | |||
| + | The machine now encounters a 1 in this default state 'A' (represented by AHL). This results in the activation of the second module which in turn produces more of ogr. This acts like a positive feedback loop and hence state 'B' is switched on and can remain active without intervention from cassette A. | ||
=Problems Associated with such an Approach= | =Problems Associated with such an Approach= | ||
Revision as of 22:05, 21 October 2009
Contents |
The Complete Turing Machine
The above image is a representation of the complete turing machine including inter state regulation. This model is true to Alan Turing's vision of multi-mutually exclusive states and the states being regulated by one another through outputs.
Working of the Construct
In the above construct, there are three independent modules that function
- A constitutively working module which produces LuxR protein and LacI and Cassette 1 (encodes ogr and activates state 'B')
- A State-'B' which is activated by State' A' and which in turn represses the working of the default state
- An AND gate regulated activation of the turing machine function (it could be lysis, expression of a fluorescent protein or the synthesis of vanillin or methyl salicylate)
The default state of the turing machine is 'A'. In this state it encounters a '0' (represented by Lactose) first.However there are no Lactose responsive elements in either the first or the second module. The third Module which has such a module however also requires the presence of the phage protein 'ogr ' . Hence the first 0 is unable to produce any effect and the machine moves on to the next cell in the tape while remaining in state A.
The machine now encounters a 1 in this default state 'A' (represented by AHL). This results in the activation of the second module which in turn produces more of ogr. This acts like a positive feedback loop and hence state 'B' is switched on and can remain active without intervention from cassette A.
