Team:IIT Bombay India/Notebook

From 2009.igem.org

(Difference between revisions)

Revision as of 08:43, 21 October 2009

Week1 FACS Trial

Week2 (May, 18-23) Preparation

Week3 (May, 25-30) FACS

Week4 (Jun, 1-6) Growth Curve

Week5 (Jun, 8-13) FACS:YFP signal

Week6 (Jun, 15-20) FACS:CFP signal

Week7 (Jun, 22-27) FACS repeat

Week8 (Jun-Jul, 29-4)Req.:Transformation

Week9 (Jul, 6-11) Cultured Host

Week10(Jul, 13-18) Transformation of 4 strains

Week11(Jul, 20-24) FACS:Constructs

Week12(Jul, 27-31) FACS

Week13(Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)

Week14(Aug, 10-15) Preparation for time course expression analysis

Week15(Aug, 17-22) FACS time course

Week16(Aug, 24-29) FACS: different IPTG conc.

Week17(Aug-Sep, 31-5)Growth curves for all the four strains

Week18(Sep, 7-13) Plasmid extraction and Gel electrophoresis

Week19(Sep, 14-20) Growth curve on Lactose (ptet A and plac K)

Week20(Sep, 21-27) Growth curve on Lactose (plac A and ptet K)

Week21(Sep-Oct, 28-3)Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)

Week22(Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)

Week23(Oct, 12-17)Growth curve on Lactose (ptet A and plac K)

Week24(Oct, 18-21)Data compilation and analysis, wiki uploads

@@ Line 1: / Line 1: @@
 [[Week1]] FACS Trial
-[[Week2]] Preparation (May, 18-23)
+[[Week2]] (May, 18-23)   Preparation
-[[Week3]] FACS (May, 25-30)
+[[Week3]] (May, 25-30)   FACS
-[[Week4]] Growth Curve (Jun, 1-6)
+[[Week4]] (Jun, 1-6)     Growth Curve
-[[Week5]] FACS:YFP signal (Jun, 8-13)
+[[Week5]] (Jun, 8-13)    FACS:YFP signal
-[[Week6]] FACS:CFP signal (Jun, 15-20)
+[[Week6]] (Jun, 15-20)   FACS:CFP signal
-[[Week7]] FACS repeat (Jun, 22-27)
+[[Week7]] (Jun, 22-27)   FACS repeat
-[[Week8]] Req.:Transformation (Jun 29- Jul 4)
+[[Week8]] (Jun-Jul, 29-4)Req.:Transformation
-[[Week9]] Cultured Host (Jul, 6-11)
+[[Week9]] (Jul, 6-11)    Cultured Host
-[[Week10]] Transformation of 4 strains (Jul, 13-18)
+[[Week10]](Jul, 13-18)   Transformation of 4 strains
-[[Week11]] FACS:Constructs (Jul, 20-24)
+[[Week11]](Jul, 20-24)   FACS:Constructs
-[[Week12]] FACS (Jul, 27-31)
+[[Week12]](Jul, 27-31)   FACS
-[[Week13]] Transformations in ''E.coli'' MG1655 (lac I deleted) (Aug, 3-8)
+[[Week13]](Aug, 3-8)     Transformations in ''E.coli'' MG1655 (lac I deleted)
-[[Week14]] Preparation for time course expression analysis (Aug, 10-15)
+[[Week14]](Aug, 10-15)   Preparation for time course expression analysis
-[[Week15]] FACS time course (Aug, 17-22)
+[[Week15]](Aug, 17-22)   FACS time course
-[[Week16]] FACS: different IPTG conc. (Aug, 24-29)
+[[Week16]](Aug, 24-29)   FACS: different IPTG conc.
-[[Week17]] Growth curves for all the four strains (Aug-Sep, 31-5)
+[[Week17]](Aug-Sep, 31-5)Growth curves for all the four strains
-[[Week18]] Plasmid extraction and Gel electrophoresis (Sep, 7-13)
+[[Week18]](Sep, 7-13)    Plasmid extraction and Gel electrophoresis
-[[Week19]] Growth curve on Lactose (ptet A and plac K) (Sep, 14-20)
+[[Week19]](Sep, 14-20)   Growth curve on Lactose (ptet A and plac K)
-[[Week20]] Growth curve on Lactose (plac A and ptet K) (Sep, 21-27)
+[[Week20]](Sep, 21-27)   Growth curve on Lactose (plac A and ptet K)
-[[Week21]] Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)(Sep-Oct, 28-3)
+[[Week21]](Sep-Oct, 28-3)Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)
-[[Week22]] Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)(Oct, 5-10)
+[[Week22]](Oct, 5-10)    Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)
-[[Week23]] Growth curve on Lactose (ptet A and plac K) (Oct, 12-17)
+[[Week23]](Oct, 12-17)Growth curve on Lactose (ptet A and plac K)
-[[Week24]] Data compilation and analysis, wiki uploads(Oct, 18-21)
+[[Week24]](Oct, 18-21)Data compilation and analysis, wiki uploads