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Team:IIT Bombay India/Notebook
From 2009.igem.org
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[[Week10]] Transformation of 4 strains (Jul, 13-18) | [[Week10]] Transformation of 4 strains (Jul, 13-18) | ||
| - | [[Week11]] FACS :Constructs (Jul, 20-24) | + | [[Week11]] FACS:Constructs (Jul, 20-24) |
[[Week12]] FACS (Jul, 27-31) | [[Week12]] FACS (Jul, 27-31) | ||
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| + | [[Week13]] Transformations in ''E.coli'' MG1655 (lac I deleted) (Aug, 3-8) | ||
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| + | [[Week14]] Preparation for time course expression analysis (Aug, 10-15) | ||
| + | |||
| + | [[Week15]] FACS time course (Aug, 17-22) | ||
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| + | [[Week16]] FACS: different IPTG conc. (Aug, 24-29) | ||
| + | |||
| + | [[Week17]] Growth curves for all the four strains (Aug-Sep, 31-5) | ||
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| + | [[Week18]] Plasmid extraction and Gel electrophoresis (Sep, 7-13) | ||
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| + | [[Week19]] Growth curve on Lactose (ptet A and plac K) (Sep, 14-20) | ||
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| + | [[Week20]] Growth curve on Lactose (plac A and ptet K) (Sep, 21-27) | ||
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| + | [[Week21]] Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)(Sep-Oct, 28-3) | ||
| + | |||
| + | [[Week22]] Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)(Oct, 5-10) | ||
| + | |||
| + | [[Week23]] Growth curve on Lactose (ptet A and plac K) (Oct, 12-17) | ||
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| + | [[Week24]] Data compilation and analysis, wiki uploads(Oct, 18-21) | ||
Revision as of 05:33, 21 October 2009
Week1 FACS Trial
Week2 Preparation (May, 18-23)
Week3 FACS (May, 25-30)
Week4 Growth Curve (Jun, 1-6)
Week5 FACS:YFP signal (Jun, 8-13)
Week6 FACS:CFP signal (Jun, 15-20)
Week7 FACS repeat (Jun, 22-27)
Week8 Req.:Transformation (Jun 29- Jul 4)
Week9 Cultured Host (Jul, 6-11)
Week10 Transformation of 4 strains (Jul, 13-18)
Week11 FACS:Constructs (Jul, 20-24)
Week12 FACS (Jul, 27-31)
Week13 Transformations in E.coli MG1655 (lac I deleted) (Aug, 3-8)
Week14 Preparation for time course expression analysis (Aug, 10-15)
Week15 FACS time course (Aug, 17-22)
Week16 FACS: different IPTG conc. (Aug, 24-29)
Week17 Growth curves for all the four strains (Aug-Sep, 31-5)
Week18 Plasmid extraction and Gel electrophoresis (Sep, 7-13)
Week19 Growth curve on Lactose (ptet A and plac K) (Sep, 14-20)
Week20 Growth curve on Lactose (plac A and ptet K) (Sep, 21-27)
Week21 Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)(Sep-Oct, 28-3)
Week22 Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)(Oct, 5-10)
Week23 Growth curve on Lactose (ptet A and plac K) (Oct, 12-17)
Week24 Data compilation and analysis, wiki uploads(Oct, 18-21)
