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Team:IIT Bombay India/Notebook
From 2009.igem.org
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[[Week1]] FACS Trial | [[Week1]] FACS Trial | ||
| - | [[Week2]] (May, 18-23) | + | [[Week2]] (May, 18-23) Preparation |
| - | [[Week3]] (May, 25-30) | + | [[Week3]] (May, 25-30) FACS |
| - | [[Week4]] (Jun, 1-6) | + | [[Week4]] (Jun, 1-6) Growth Curve |
| - | [[Week5]] (Jun, 8-13) | + | [[Week5]] (Jun, 8-13) FACS:YFP signal |
| - | [[Week6]] (Jun, 15-20) | + | [[Week6]] (Jun, 15-20) FACS:CFP signal |
| - | [[Week7]] (Jun, 22-27) | + | [[Week7]] (Jun, 22-27) FACS repeat |
| - | [[Week8]] (Jun-Jul, 29-4)Req.:Transformation | + | [[Week8]] (Jun-Jul, 29-4) Req.:Transformation |
| - | [[Week9]] (Jul, 6-11) | + | [[Week9]] (Jul, 6-11) Cultured Host |
| - | [[Week10]](Jul, 13-18) | + | [[Week10]] (Jul, 13-18) Transformation of 4 strains |
| - | [[Week11]](Jul, 20-24) | + | [[Week11]] (Jul, 20-24) FACS:Constructs |
| - | [[Week12]](Jul, 27-31) | + | [[Week12]] (Jul, 27-31) FACS |
| - | [[Week13]](Aug, 3-8) | + | [[Week13]] (Aug, 3-8) Transformations in ''E.coli'' MG1655 (lac I deleted) |
| - | [[Week14]](Aug, 10-15) | + | [[Week14]] (Aug, 10-15) Preparation for time course expression analysis |
| - | [[Week15]](Aug, 17-22) | + | [[Week15]] (Aug, 17-22) FACS time course |
| - | [[Week16]](Aug, 24-29) | + | [[Week16]] (Aug, 24-29) FACS: different IPTG conc. |
| - | [[Week17]](Aug-Sep, 31-5)Growth curves for all the four strains | + | [[Week17]] (Aug-Sep, 31-5) Growth curves for all the four strains |
| - | [[Week18]](Sep, 7-13) | + | [[Week18]] (Sep, 7-13) Plasmid extraction and Gel electrophoresis |
| - | [[Week19]](Sep, 14-20) | + | [[Week19]] (Sep, 14-20) Growth curve on Lactose (ptet A and plac K) |
| - | [[Week20]](Sep, 21-27) | + | [[Week20]] (Sep, 21-27) Growth curve on Lactose (plac A and ptet K) |
| - | [[Week21]](Sep-Oct, 28-3)Growth curve on Lactose varying IPTG concentrations (ptet A and plac K) | + | [[Week21]] (Sep-Oct, 28-3) Growth curve on Lactose varying IPTG concentrations (ptet A and plac K) |
| - | [[Week22]](Oct, 5-10) | + | [[Week22]] (Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K) |
| - | [[Week23]](Oct, 12-17)Growth curve on Lactose (ptet A and plac K) | + | [[Week23]] (Oct, 12-17) Growth curve on Lactose (ptet A and plac K) |
| - | [[Week24]](Oct, 18-21)Data compilation and analysis, wiki uploads | + | [[Week24]] (Oct, 18-21) Data compilation and analysis, wiki uploads |
Revision as of 08:46, 21 October 2009
Week1 FACS Trial
Week2 (May, 18-23) Preparation
Week3 (May, 25-30) FACS
Week4 (Jun, 1-6) Growth Curve
Week5 (Jun, 8-13) FACS:YFP signal
Week6 (Jun, 15-20) FACS:CFP signal
Week7 (Jun, 22-27) FACS repeat
Week8 (Jun-Jul, 29-4) Req.:Transformation
Week9 (Jul, 6-11) Cultured Host
Week10 (Jul, 13-18) Transformation of 4 strains
Week11 (Jul, 20-24) FACS:Constructs
Week12 (Jul, 27-31) FACS
Week13 (Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)
Week14 (Aug, 10-15) Preparation for time course expression analysis
Week15 (Aug, 17-22) FACS time course
Week16 (Aug, 24-29) FACS: different IPTG conc.
Week17 (Aug-Sep, 31-5) Growth curves for all the four strains
Week18 (Sep, 7-13) Plasmid extraction and Gel electrophoresis
Week19 (Sep, 14-20) Growth curve on Lactose (ptet A and plac K)
Week20 (Sep, 21-27) Growth curve on Lactose (plac A and ptet K)
Week21 (Sep-Oct, 28-3) Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)
Week22 (Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)
Week23 (Oct, 12-17) Growth curve on Lactose (ptet A and plac K)
Week24 (Oct, 18-21) Data compilation and analysis, wiki uploads
