Team:IIT Bombay India/Notebook

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Revision as of 08:43, 21 October 2009 by Supriyak (Talk | contribs)

Week1 FACS Trial

Week2 (May, 18-23) Preparation

Week3 (May, 25-30) FACS

Week4 (Jun, 1-6) Growth Curve

Week5 (Jun, 8-13) FACS:YFP signal

Week6 (Jun, 15-20) FACS:CFP signal

Week7 (Jun, 22-27) FACS repeat

Week8 (Jun-Jul, 29-4)Req.:Transformation

Week9 (Jul, 6-11) Cultured Host

Week10(Jul, 13-18) Transformation of 4 strains

Week11(Jul, 20-24) FACS:Constructs

Week12(Jul, 27-31) FACS

Week13(Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)

Week14(Aug, 10-15) Preparation for time course expression analysis

Week15(Aug, 17-22) FACS time course

Week16(Aug, 24-29) FACS: different IPTG conc.

Week17(Aug-Sep, 31-5)Growth curves for all the four strains

Week18(Sep, 7-13) Plasmid extraction and Gel electrophoresis

Week19(Sep, 14-20) Growth curve on Lactose (ptet A and plac K)

Week20(Sep, 21-27) Growth curve on Lactose (plac A and ptet K)

Week21(Sep-Oct, 28-3)Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)

Week22(Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)

Week23(Oct, 12-17)Growth curve on Lactose (ptet A and plac K)

Week24(Oct, 18-21)Data compilation and analysis, wiki uploads