Team:IIT Madras/Notebook/Sandbox

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(Intermediates and final constructs planned for the the 2 plasmid locking system)
(Intermediates and final constructs planned for the the 2 plasmid locking system)
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== '''Intermediates and final constructs planned for the the 2 plasmid locking system''' ==
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== '''Intermediates and proposed constructs for the the 2 plasmid locking system''' ==
== Original design ==
== Original design ==

Revision as of 17:31, 18 October 2009

Contents

Intermediates and proposed constructs for the the 2 plasmid locking system

Original design

In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).


2plasmidoriginaldesign.jpg




Final design

We decided to remove the extra antibiotic markers from the inserts. Then it looks like this


File:2plasmidfinaldesign.jpg


We had more than one strategy to build the whole construct.


strategy 1

File:2plasmidstrategy1.jpg



strategy 2

File:2plasmidstrategy2.jpg


Intermediates and final constructs planned for the the 3 plasmid locking system

intermediates planned through PCR protocol

Plasmids for a self locking assembly