Team:IIT Madras/Notebook/Sandbox

From 2009.igem.org

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(Original design)
(Final design)
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'''Plasmid 1'''
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We had more than one strategy to build the whole construct.
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'''Plasmid 2'''
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We had more than one strategy to build the whole construct.
== ''strategy 1'' ==
== ''strategy 1'' ==

Revision as of 13:40, 19 October 2009

Contents

Intermediates and proposed constructs for the the 2 plasmid locking system

Original design

In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).


Plasmid 1


2plasmidoriginaldesignplasmid1.jpg


Plasmid 2


2plasmidoriginaldesignplasmid2.jpg


Final design

We decided to remove the extra antibiotic markers from the inserts. Then it looks like this


Plasmid 1


2plasmidfinaldesignplasmid1.jpg


Plasmid 2


2plasmidfinaldesignplasmid2.jpg


We had more than one strategy to build the whole construct.

strategy 1

File:2plasmidstrategy1.jpg



strategy 2

File:2plasmidstrategy2.jpg


Intermediates and proposed constructs for the the 3 plasmid locking system

We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.

intermediates planned through PCR protocol

Plasmids for a self locking assembly