Team:IIT Madras/Results

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Indian Institute of Technology,Madras

Results

Comparing the differences in the growth rates of cells with and without plasmids in various media

Growth curves

Fig 8.1: DH5a grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.

Fig 8.2: RFP (pSB1C3) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.

Fig 8.3: CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.

Fig 8.4: RFP (pSB1C3) - CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.

Fig 8.5: All 4 strains in media without antibiotics.

Fig 8.6: All 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.

Fig 8.7: log (OD600) vs Time plot for all 4 strains in media without antibiotics.

Fig 8.7: log (OD600) vs Time plot for all 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.

Modeling

Fluorescent Imaging

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 [[Image:DH5aall4cases.jpg|600px]]
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-<html>><b><font color="#000">Fig 8.1: </font></b><i>DH5a grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.</i></html>
+<html><b><font color="#000">Fig 8.1: </font></b><i>DH5a grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.</i></html>
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-RFP (pSB1C3) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.
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-CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.
+<html><b><font color="#000">Fig 8.2: </font></b><i>RFP (pSB1C3) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.</i></html>
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-RFP (pSB1C3) - CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.
+<html><b><font color="#000">Fig 8.3: </font></b><i>CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.</i></html>
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 [[Image:RC4cases.jpg|600px]]
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+<html><b><font color="#000">Fig 8.4: </font></b><i>RFP (pSB1C3) - CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.</i></html>
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-All 4 strains in media without antibiotics
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-All 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.
+<html><b><font color="#000">Fig 8.5: </font></b><i>All 4 strains in media without antibiotics.</i></html>
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 [[Image:corraball4.jpg|600px]]
-<html><br></html> <html><br></html>
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-log (OD600) vs Time plot for all 4 strains in media without antibiotics
+<html><b><font color="#000">Fig 8.6: </font></b><i>All 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.</i></html>
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-<html><br></html><html><br></html>
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-log (OD600) vs Time plot for all 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.
+<html><b><font color="#000">Fig 8.7: </font></b><i>log (OD600) vs Time plot for all 4 strains in media without antibiotics.</i></html>
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-<html><br></html><html><br></html>
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+<html><b><font color="#000">Fig 8.7: </font></b><i>log (OD600) vs Time plot for all 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.</i></html>
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 ===Modeling===