Team:IPN-UNAM-Mexico/Notebook/August 8 2009

From 2009.igem.org

(Difference between revisions)
(New page: August 8 2009)
Line 1: Line 1:
-
August 8 2009
+
<HTML><HEAD>
 +
<TITLE></TITLE>
 +
<meta http-equiv="Content-Style-Type" content="text/css">
 +
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
 +
<LINK HREF="style.css" TYPE="text/css" REL="stylesheet">
 +
 
 +
<script type="text/javascript">
 +
<!--
 +
function MM_findObj(n, d) { //v4.01
 +
  var p,i,x;  if(!d) d=document; if((p=n.indexOf("?"))>0&&parent.frames.length) {
 +
    d=parent.frames[n.substring(p+1)].document; n=n.substring(0,p);}
 +
  if(!(x=d[n])&&d.all) x=d.all[n]; for (i=0;!x&&i<d.forms.length;i++) x=d.forms[i][n];
 +
  for(i=0;!x&&d.layers&&i<d.layers.length;i++) x=MM_findObj(n,d.layers[i].document);
 +
  if(!x && d.getElementById) x=d.getElementById(n); return x;
 +
}
 +
function MM_preloadImages() { //v3.0
 +
  var d=document; if(d.images){ if(!d.MM_p) d.MM_p=new Array();
 +
    var i,j=d.MM_p.length,a=MM_preloadImages.arguments; for(i=0; i<a.length; i++)
 +
    if (a[i].indexOf("#")!=0){ d.MM_p[j]=new Image; d.MM_p[j++].src=a[i];}}
 +
}
 +
function MM_swapImgRestore() { //v3.0
 +
  var i,x,a=document.MM_sr; for(i=0;a&&i<a.length&&(x=a[i])&&x.oSrc;i++) x.src=x.oSrc;
 +
}
 +
function MM_swapImage() { //v3.0
 +
  var i,j=0,x,a=MM_swapImage.arguments; document.MM_sr=new Array; for(i=0;i<(a.length-2);i+=3)
 +
  if ((x=MM_findObj(a[i]))!=null){document.MM_sr[j++]=x; if(!x.oSrc) x.oSrc=x.src; x.src=a[i+2];}
 +
}
 +
//-->
 +
</script>
 +
<style type="text/css">
 +
<!--
 +
.style7 {color: #FFFFFF}
 +
.style8 {
 +
font-size: 12
 +
}
 +
.style11 {font-size: 12; color: #22405A; font-weight: bold; }
 +
-->
 +
</style>
 +
</HEAD>
 +
<BODY onLoad="MM_preloadImages('https://static.igem.org/mediawiki/2009/f/f5/Home.jpg','https://static.igem.org/mediawiki/2009/6/6c/ModelingMX.jpg','https://static.igem.org/mediawiki/2009/6/61/PartsMX.jpg','https://static.igem.org/mediawiki/2009/c/c8/Project2MX.jpg','https://static.igem.org/mediawiki/2009/4/46/TeamMX.jpg','https://static.igem.org/mediawiki/2009/8/85/CollaborationMX.jpg')" >
 +
<br style="line-height:7px">
 +
<table cellspacing="0" cellpadding="0" align="center">
 +
  <tr>
 +
  <td style="width:750px; height:800px">
 +
<table cellspacing="0" cellpadding="0">
 +
  <tr>
 +
<td style="width:30px; height:800px "></td>
 +
<td style="width:688px; height:800px ">
 +
<table cellspacing="0" cellpadding="0">
 +
  <tr>
 +
<td width="937" style="width:688px; height:275px ">
 +
    <table cellspacing="0" cellpadding="0">
 +
  <tr>
 +
<td style="width:688px; height:73px ">
 +
<br style="line-height:22px">
 +
<table cellspacing="0" cellpadding="0">
 +
  <tr>
 +
<td style="width:553px; height:51px "><img src="https://static.igem.org/mediawiki/2009/7/7f/México44MX.png" alt="" width="45" height="45" hspace="15"><a href="http://www.unam.mx/" class="style7"><img src="https://static.igem.org/mediawiki/2009/8/8a/UNAMMX.gif" alt="UNAM" width="50" height="56" hspace="50"></a></a><a href="http://www.fciencias.unam.mx/" class="style7"><img src="https://static.igem.org/mediawiki/2009/6/6e/FCienciasMX.png" alt="FCIENCIAS" width="50" height="57" hspace="50"></a><a href="http://www.ira.cinvestav.mx/" class="style7"><img src="https://static.igem.org/mediawiki/2009/c/ce/CinvestavMX.gif" alt="CINVESTAV" width="50" height="62" hspace="50"></a></a></td>
 +
<td style="width:135px; height:51px; vertical-align:bottom; text-align:right  "><a href="https://2009.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2009/0/0c/HomeMX.gif" alt="" border="0"></a><a href="#"><img src="https://static.igem.org/mediawiki/2009/b/b2/ContactMX.gif" alt="" hspace="3" border="0" ></a></td>
 +
  </tr>
 +
</table> </td>
 +
  </tr>
 +
  <tr>
 +
<td align="center" valign="middle" class="head" style="width:688px; height:163px ">
 +
<img src="https://static.igem.org/mediawiki/2009/e/e2/BannerM%C3%A9xico.jpg" alt="BannerMX" width="688" height="161" align="absmiddle"><br style="line-height:55px"></td>
 +
  </tr>
 +
  <tr>
 +
<td width="50" height="65" class="tall_menu" style="width:688px; height:39px "><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('HOME','','https://static.igem.org/mediawiki/2009/f/f5/Home.jpg',1)"><img src="https://static.igem.org/mediawiki/2009/e/ec/Home2mx.jpg" alt="Home" name="HOME" width="113" height="32" border="0"></a><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Project" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('PROJECT','','https://static.igem.org/mediawiki/2009/c/c8/Project2MX.jpg',1)"><img src="https://static.igem.org/mediawiki/2009/5/5e/ProjectMX.jpg" alt="The Project" name="PROJECT" width="113" height="32" hspace="1" border="0"></a><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Team" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('TEAM','','https://static.igem.org/mediawiki/2009/4/46/TeamMX.jpg',1)"><img src="https://static.igem.org/mediawiki/2009/0/05/Team2MX.jpg" alt="The Team" name="TEAM" width="113" height="32" hspace="1" border="0"></a><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Modeling" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('MODELING','','https://static.igem.org/mediawiki/2009/6/6c/ModelingMX.jpg',1)"><img src="https://static.igem.org/mediawiki/2009/a/a1/Modeling2MX.jpg" alt="Modeling" name="MODELING" width="113" height="32" hspace="1" border="0"></a><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Parts" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('PARTS','','https://static.igem.org/mediawiki/2009/6/61/PartsMX.jpg',1)"><img src="https://static.igem.org/mediawiki/2009/f/f0/Parts2MX.jpg" alt="Parts" name="PARTS" width="113" height="32" hspace="1" border="0"></a><a href="https://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Collaboration&action=edit&redlink=1" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('COLLABORATION','','https://static.igem.org/mediawiki/2009/8/85/CollaborationMX.jpg',1)"><img src="https://static.igem.org/mediawiki/2009/3/33/Collaboration2MX.jpg" alt="Collaboration" name="COLLABORATION" width="113" height="32" border="0"></a>
 +
  <table width="100%" height=100%" "border="0" cellspacing="0" cellpadding="0">
 +
                                <tr>
 +
                                  <td><p>&nbsp;</p>
 +
                                    <p align="left" class="style8"><strong>August 8th, 2009</strong></p>
 +
                                    <p align="left" class="style8"><br>
 +
                                    6 Biobricks were ligated (see 06/08/09) and they grew in the following  plates:</p>
 +
                                    <ul>
 +
                                      <li>15: 3 lig2 Amp/Kan</li>
 +
                                      <li>2:3 lig1 Amp</li>
 +
                                      <li>2:3 lig2</li>
 +
                                    </ul>
 +
                                    <blockquote>
 +
                                      <p align="left" class="style8">&nbsp;</p>
 +
                                    </blockquote>
 +
                                    <p align="left" class="style8">Falcon tubes were inoculated with colonies picked up from the ones that  grew in the plates. We took 2 from 15:13 and 2 from 2:3 with a total of 4 Falcon  tubes with their respective antibiotic resistances.</p>
 +
<p align="left" >&nbsp;</p>
 +
                                    <p align="left" >&nbsp;</p>
 +
                                    <table width="100%" height="108" border="0" align="left">
 +
                                      <tr>
 +
                                        <td width="50%"><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Notebook/August_7_2009#August_7th"><img src="https://static.igem.org/mediawiki/2009/3/38/AnteriorMX.JPG" alt="Previous" width="70" height="70" align="left" border="0"></a> </td>
 +
                                        <td width="50%" height="104"> <a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Notebook/August_9_2009"><img src="https://static.igem.org/mediawiki/2009/3/3a/SiguienteMX.JPG" alt="Next" width="70" height="70" align="right" border="0"></a></td>
 +
                                      </tr>
 +
                                    </table>
 +
                                    <p align="left">&nbsp;</p>
 +
                                    <p align="left">&nbsp;</p>
 +
                                    <p align="left">&nbsp;</p>
 +
                                    <p align="left">&nbsp;</p>                                  </td>
 +
                                </tr>
 +
                              </table></td>
 +
    </tr>
 +
  </table> </td>
 +
  </tr>
 +
  <tr>
 +
<td style="width:688px; height:416px ">&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
<td style="width:688px; height:109px ">
 +
<table cellspacing="0" cellpadding="0" bordercolor="#FFFFFF" " class="foot" >
 +
  <tr>
 +
<td height="105" style="width:22px; height:54px "></td>
 +
<td style="width:496px; height:54px; vertical-align:middle " >&nbsp;</td>
 +
<td align="center" valign="middle" style="width:169px; height:54px; text-align:right ">
 +
  <p><script type="text/javascript">
 +
var sc_project=5020442;
 +
var sc_invisible=0;
 +
var sc_partition=57;
 +
var sc_click_stat=1;
 +
var sc_security="8325b5da";
 +
</td>
 +
  </tr>
 +
</table> </td>
 +
  </tr>
 +
</table>
 +
</td>
 +
<td style="width:32px; height:800px "></td>
 +
  </tr>
 +
</table>
 +
 +
 +
</td>
 +
  </tr>
 +
</table>
 +
 
 +
</BODY>
 +
</HTML>

Revision as of 06:52, 14 October 2009



UNAMFCIENCIASCINVESTAV
BannerMX
HomeThe ProjectThe TeamModelingPartsCollaboration

 

August 8th, 2009


6 Biobricks were ligated (see 06/08/09) and they grew in the following plates:

  • 15: 3 lig2 Amp/Kan
  • 2:3 lig1 Amp
  • 2:3 lig2

 

Falcon tubes were inoculated with colonies picked up from the ones that grew in the plates. We took 2 from 15:13 and 2 from 2:3 with a total of 4 Falcon tubes with their respective antibiotic resistances.

 

 

Previous Next