Team:IPN-UNAM-Mexico/Notebook/July 7 2009

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Revision as of 01:45, 21 September 2009



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Digestion of the following Biobricks with the indicated enzimes:

 

 
Biobrick
Order
Enzime

R0079

Prefix

E, S

F1610

Sufix

E, X

B0034

Prefix

E, S

C0078

Sufix

E, X

B0034

Prefix

E, S

C0079

Sufix

E, X

B0015

Sufix

E, X
 

 

  Note: E=EcoR I, S=Spe I, X=Xba I

 


 

 

 

 

Cuts were done thinking on the standard assembling 10 with the 2-way-ligation.

 

Procedement:

- Take the purified DNA stocks from the previous Biobricks
- Take the enzimes and add 100 μl of Buffer

2 μl of each sample were analized on a Spectrophotometer to determine the concentration of DNA on each Biopart.

 

 

Biobrick

Concentration (ng/μl)

F1610

158.9

B0015

11.5

C0079

7.5

B0034

11.6

C0078

7.7

B0079

11.2

 

 

 

 

 

 

 

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