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Team:IPN-UNAM-Mexico/Parts
From 2009.igem.org
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==[[Image:Month-icon.png | 50px]]Biobricks== | ==[[Image:Month-icon.png | 50px]]Biobricks== | ||
| - | + | We contribute to the registry with 11 new biobricks, 5 of them are favorite biobricks from the team and in this section we document them: | |
===Main biobricks: modules and favorites=== | ===Main biobricks: modules and favorites=== | ||
====Modules==== | ====Modules==== | ||
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<center> | <center> | ||
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! Type | ! Type | ||
! Image | ! Image | ||
| + | ! Description | ||
|- | |- | ||
| '''Mod 1''' | | '''Mod 1''' | ||
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| '''Las AHL''' | | '''Las AHL''' | ||
| [[Image:AI LasI.jpg|400px|left|Las AHL]] | | [[Image:AI LasI.jpg|400px|left|Las AHL]] | ||
| + | | BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator | ||
|- | |- | ||
| '''Mod 2''' | | '''Mod 2''' | ||
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| '''Lac inverter''' | | '''Lac inverter''' | ||
| [[Image:AI Lac.jpg|500px|left|Lac Inverter]] | | [[Image:AI Lac.jpg|500px|left|Lac Inverter]] | ||
| + | | Constitutive promoter J23100 with Lac system inverter controlling the expression of LasR | ||
|- | |- | ||
| '''Mod 3''' | | '''Mod 3''' | ||
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| '''Tet inverter''' | | '''Tet inverter''' | ||
| [[Image:AI Tet.jpg|500px|left|link=https://2009.igem.org/Team:IPN-UNAM-Mexico/Parts]] | | [[Image:AI Tet.jpg|500px|left|link=https://2009.igem.org/Team:IPN-UNAM-Mexico/Parts]] | ||
| + | | J23100 constitutive promoter directs the expression of tetracycline repressor (TetR). TetR binds to pTet regulatory region resulting in a negative control of the production of LuxR. The whole system acts as an Inverter of Tet system controlling LuxR expression. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. | ||
|- | |- | ||
| '''Mod 4''' | | '''Mod 4''' | ||
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| '''Lux AHL''' | | '''Lux AHL''' | ||
| [[Image:AI LuxI.jpg|300px|left|Lux AHL]] | | [[Image:AI LuxI.jpg|300px|left|Lux AHL]] | ||
| + | | This biobrick has a promoter inducible by PAI+LasR (BBa_R0079) I.e. positive regulation and produces LuxI enzyme (BBa_F1610). This enzyme produces 3OC6HSL (AI). | ||
|} | |} | ||
</center> | </center> | ||
Revision as of 00:51, 22 October 2009
Biobricks
Biobricks
We contribute to the registry with 11 new biobricks, 5 of them are favorite biobricks from the team and in this section we document them:
Main biobricks: modules and favorites
Modules
| Module | Biobrick Name | Type | Image | Description |
|---|---|---|---|---|
| Mod 1 | BBa_K266006 | Las AHL |  | BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator |
| Mod 2 | BBa_K266004 | Lac inverter |  | Constitutive promoter J23100 with Lac system inverter controlling the expression of LasR |
| Mod 3 | BBa_K266001 | Tet inverter |  | J23100 constitutive promoter directs the expression of tetracycline repressor (TetR). TetR binds to pTet regulatory region resulting in a negative control of the production of LuxR. The whole system acts as an Inverter of Tet system controlling LuxR expression. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. |
| Mod 4 | BBa_K266000 | Lux AHL |  | This biobrick has a promoter inducible by PAI+LasR (BBa_R0079) I.e. positive regulation and produces LuxI enzyme (BBa_F1610). This enzyme produces 3OC6HSL (AI). |
Favorites
| Biobrick Name | Image |
|---|---|
| BBa_K266007 |  |
| BBa_K2660010 |  |
Auxiliary biobricks
| Biobrick Name | Image |
|---|---|
| BBa_K266002 |  |
| BBa_K266005 |  |
| BBa_K266008 |  |
| BBa_K266009 |  |
| BBa_K2660011 |  |
Plasmids
