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Team:IPN-UNAM-Mexico/Parts
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Revision as of 01:01, 22 October 2009 by LUIS DE JESUS (Talk | contribs)
Biobricks
Biobricks
We contribute to the registry with 11 new biobricks, 5 of them are favorite biobricks from the team and in this section we document them:
Main biobricks: modules and Projects
Modules
| Module and Status | Biobrick Name | Type | Image | Description |
|---|---|---|---|---|
| Mod 1 Sent | BBa_K266006 | Las AHL |  | BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator. |
| Mod 2 Sent | BBa_K266004 | Lac inverter |  | Constitutive promoter J23100 with Lac system inverter controlling the expression of LasR. |
| Mod 3 Sent | BBa_K266001 | Tet inverter |  | J23100 constitutive promoter directs the expression of tetracycline repressor (TetR). TetR binds to pTet regulatory region resulting in a negative control of the production of LuxR. The whole system acts as an Inverter of Tet system controlling LuxR expression. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. |
| Mod 4 Sent | BBa_K266000 | Lux AHL |  | This biobrick has a promoter inducible by PAI+LasR (BBa_R0079) I.e. positive regulation and produces LuxI enzyme (BBa_F1610). This enzyme produces 3OC6HSL (AI). |
Projects
| Status | Biobrick Name | Image | Description |
|---|---|---|---|
| Sent | BBa_K266007 |  | Complex Quorum sensing circuit that receives the signal of PAI+LasR and AI+LuxR to control the production of LuxI and LasI enzymes. |
| Pending | BBa_K2660010 |  | Tet constitutive inverter controlling LasR expression and Lac constitutive inverter controlling LuxR expression. |
Auxiliary biobricks
| Status | Biobrick Name | Image | Description |
|---|---|---|---|
| Sent | BBa_K266002 |  | LasR coding region and a double terminator. |
| Sent | BBa_K266005 |  | BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the expresion of LasI enzyme, no terminator. |
| Sent | BBa_K266008 |  | The BBa_J23100 constitutive promoter with Lac systems inverter. |
| Sent | BBa_K266009 |  | The BBa_J23100 constitutive promoter with Lac systems inverter and strong RBS. |
| Sent | BBa_K2660011 |  | POPS regulated and Lac inverter system with strong RBS. |
Plasmids
