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Team:Illinois/Hybrid Promoter
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BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them. | BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them. | ||
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| + | == '''July 13''' == | ||
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| + | Concerns: We want the sRNA gene on a high copy plasmid and the target sequence on low copy plasmid. This could require up to 7 different plasmids to transform into the cell. We need to look into the feasibility of this. | ||
Revision as of 15:29, 13 July 2009
Hybrid Promoter
Goals: The goal of this side-project is the create a hybrid or combinatorial promoter that accepts two inputs. This will be used in the creation of an AND logic gate.
Papers of Interest:
Engineered gene circuits Jeff Hasty, David McMillen & J. J. Collins
Construction and Enhancement of a Minimal Genetic AND Logic Gate Daniel J. Sayut, Yan Niu, and Lianhong Sun
Combinatorial Synthesis of Genetic Networks Cabrevelin C. Guet, Michael B. Elowitz, Weihong Hsing, Stanislas Leibler
Programming gene expression with combinatorial promoters Robert Sidney Cox, III, Michael G Surette, and Michael B Elowitz
Biobricks of Interest:
BBa_K091101 pTet_Lac hybrid promoter
BBa_R0010 lacl regulated promoter
BBa_R0040 TetR repressible promoter
BBa_K137125 LacI-repressed promoter B4
July 10
Today we came up with a schematic of a possible decoder:
We looked more closely at the biobricks: BBa_I14032, BBa_R0040, BBa_K137125, BBa_K091101.
BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them.
July 13
Concerns: We want the sRNA gene on a high copy plasmid and the target sequence on low copy plasmid. This could require up to 7 different plasmids to transform into the cell. We need to look into the feasibility of this.
