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Team:Illinois/MicA
From 2009.igem.org
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We used PCR to extract the sRNA gene: MicA and target sequence : ompA. from the e.coli chromosome. We then ran a gel to make sure that we had the right DNA fragments. Our results corresponded to our predictions. | We used PCR to extract the sRNA gene: MicA and target sequence : ompA. from the e.coli chromosome. We then ran a gel to make sure that we had the right DNA fragments. Our results corresponded to our predictions. | ||
| - | [[Image: | + | [[Image:illinoisgelofmicA/OmpA.jpg]] |
=='''June 17'''== | =='''June 17'''== | ||
We are completing a digestion of MicA, OmpA, and OmpF. Following the digestion we will incubate with SAP and then run a gel to verify the digestion. We will then extract the DNA for the sRNA target sequence and sRNA gene. | We are completing a digestion of MicA, OmpA, and OmpF. Following the digestion we will incubate with SAP and then run a gel to verify the digestion. We will then extract the DNA for the sRNA target sequence and sRNA gene. | ||
Revision as of 16:17, 17 June 2009
Contents |
MicA Target-GFP Fusion
Purpose:
Protocol(s) Used: (links to protocols page)
Recipe(s) Used:
Primers Used:
June 12
Journal entry text goes here.
June 16
We used PCR to extract the sRNA gene: MicA and target sequence : ompA. from the e.coli chromosome. We then ran a gel to make sure that we had the right DNA fragments. Our results corresponded to our predictions.
File:IllinoisgelofmicA/OmpA.jpg
June 17
We are completing a digestion of MicA, OmpA, and OmpF. Following the digestion we will incubate with SAP and then run a gel to verify the digestion. We will then extract the DNA for the sRNA target sequence and sRNA gene.
