Team:Illinois/MicA

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MicA Target-GFP Fusion

Purpose:

Protocol(s) Used: (links to protocols page)

Recipe(s) Used:

Primers Used:

June 12

Journal entry text goes here.

June 16

We used PCR to extract the sRNA gene: MicA and target sequence : ompA. from the e.coli chromosome. We then ran a gel to make sure that we had the right DNA fragments. Our results corresponded to our predictions.

File:IllinoisgelofmicA/OmpA.jpg

June 17

We are completing a digestion of MicA, OmpA, and OmpF. Following the digestion we will incubate with SAP and then run a gel to verify the digestion. We will then extract the DNA for the sRNA target sequence and sRNA gene.