Team:Illinois/MicC

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[https://2009.igem.org/Team:Illinois/sRNA_Library Back to sRNA Library Team page]
== '''MicC Target-GFP Fusion''' ==
== '''MicC Target-GFP Fusion''' ==

Revision as of 19:04, 26 June 2009

Click to go to the Illinois home page



Back to sRNA Library Team page

Contents

MicC Target-GFP Fusion

Primers Used:

June 15

PCRs of sRNA genes and target sequences were performed using E. coli extracted chromosomal DNA and primers. We were able to purify the ompC gene on a 3% agarose gel, however no band was visible from the micC PCR product.

June 16

Another PCR of the micC gene was done. Results should be analyzed tomorrow.

June 17

We performed a restriction enzyme digest of our small RNA and its target sequence. We then ran a gel to purify the DNA.