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This page will include periodic entries during our research. We will be documenting the progression of our project, any problems or obstacles encountered, and solutions to problems.

Browse by Lab Team

Click on a group name below to view notebook entries and information pertaining to one of our individual lab teams.

Post-Summer Progress

This section includes entries for lab work done after the summer was over.

September 24

PCRed all target sequences w/ BB sRNAs w/ no results

September 25

PCRed sRNA genes (MicC, MicA, MicF) and their respective target sequences (OmpC, OmpA, OmpF). The gel was run with the Vogel primers as a control since the previous attempt to PCR Biobricks failed.

Also PCRed plasmid pSB1A2. Used 56°C for annealing temperature instead of 58°C and got better results.

Also restreaked plates of E. coli containing plasmid PJU-334 and Biobricks BBa_K091101, BBa_I0500, and BBa_R0040.

September 26

PCRed sRNA target sequences OmpC, OmpA, OmpF, FtsZ, PtsG, GalK,. For PCRs, used

  • 4 μL chromosomal template
  • 0.4 μL of each primer
  • 5 μL 10x buffer
  • 0.8 μL Pfu polymerase
  • 1 μL dNTP mix
  • 38.4 μL dH2O

Also digested OmpC, OmpA, and OmpF target sequences from yesterday's PCR using

  • 1 μL EcoRI-HF (20,000 U/mL)
  • 3.5 μL 10x Buffer 4
  • 30 μL DNA
  • 0.35 μL BSA
  • 0.15 μL dH2O

Also cultured cells containing plasmid PJU-334 and Biobricks BBa_K091101, BBa_I0500, and BBa_R0040 overnight.

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