http://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&feed=atom&action=historyTeam:Illinois/Project - Revision history2024-03-28T11:06:31ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&diff=165459&oldid=prevReif: /* Introduction */2009-10-22T02:27:49Z<p><span class="autocomment">Introduction</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:27, 22 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 11:</td>
<td colspan="2" class="diff-lineno">Line 11:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>One such logic circuit is known as a n-to-2<html><sup>n</sup></html> binary decoder. A binary decoder is a device that receives n binary inputs (each input is either "on" or "off") and decodes them to produce one of 2<html><sup>n</sup></html> unique outputs. For example, a 2-to-4 binary decoder has two binary inputs and four different outputs. Each output can be represented in terms of a combination of the two binary inputs. For example, in a two-to-four decoder, the inputs are represented as 00, 01, 10, and 11, where each input can be 0 ("off") or 1 ("on"), the first digit corresponds to the state of the first input, and the second digit corresponds to the state of the second input. <del class="diffchange diffchange-inline">Other sizes of binary </del>decoders <del class="diffchange diffchange-inline">include </del>3-to-8 and 4-to-16. Binary decoders are widely used in computers for random-access memory and for converting microcode into control signals.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>One such logic circuit is known as a n-to-2<html><sup>n</sup></html> binary decoder. A binary decoder is a device that receives n binary inputs (each input is either "on" or "off") and decodes them to produce one of 2<html><sup>n</sup></html> unique outputs. For example, a 2-to-4 binary decoder has two binary inputs and four different outputs. Each output can be represented in terms of a combination of the two binary inputs. For example, in a two-to-four decoder, the inputs are represented as 00, 01, 10, and 11, where each input can be 0 ("off") or 1 ("on"), the first digit corresponds to the state of the first input, and the second digit corresponds to the state of the second input. <ins class="diffchange diffchange-inline">Larger </ins>decoders <ins class="diffchange diffchange-inline">such as </ins>3-to-8 and 4-to-16 <ins class="diffchange diffchange-inline">are also possible</ins>. Binary decoders are widely used in computers for random-access memory and for converting microcode into control signals.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Srna.jpg|thumb|sRNAs can be either ''cis''(A) or ''trans''(B). They can prevent protein synthesis from an mRNA by several methods, including binding to the RBS and preventing ribosome binding or binding to the 5' UTR and recruiting degradative enzymes. Some sRNAs positively regulate a gene by binding to a folded mRNA and revealing the RBS.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Srna.jpg|thumb|sRNAs can be either ''cis''(A) or ''trans''(B). They can prevent protein synthesis from an mRNA by several methods, including binding to the RBS and preventing ribosome binding or binding to the 5' UTR and recruiting degradative enzymes. Some sRNAs positively regulate a gene by binding to a folded mRNA and revealing the RBS.]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our team has chosen to create a 2-to-4 binary decoder in ''Escherichia coli''. Our novel bacteria will be able to sense concentrations of two inputs (such as two different sugars), identify these concentrations as "on" or "off", and produce one of four unique outputs (fluorescent proteins) depending on the combination of inputs. Thus, our four outputs can be represented in terms of our binary inputs as 00, 01, 10, and 11, where 0 corresponds to the "off" state (low concentration), 1 corresponds to the "on" state (high concentration), the first digit corresponds to the state of the first input, and the second digit corresponds to the state of the second input.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our team has chosen to create a 2-to-4 binary decoder in ''Escherichia coli''. Our novel bacteria will be able to sense concentrations of two inputs (such as two different sugars), identify these concentrations as "on" or "off", and produce one of four unique outputs (<ins class="diffchange diffchange-inline">we chose to use </ins>fluorescent proteins) depending on the combination of inputs. Thus, our four outputs can be represented in terms of our binary inputs as 00, 01, 10, and 11, where 0 corresponds to the "off" state (low concentration), 1 corresponds to the "on" state (high concentration), the first digit corresponds to the state of the first input, and the second digit corresponds to the state of the second input.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>One feature of our bacterial decoder will be that it will be modular. In other words, it <del class="diffchange diffchange-inline">will </del>be <del class="diffchange diffchange-inline">very simple </del>to change the two inputs that our decoder will respond to. Our design uses only two promoters that are each sensitive to a specific input. Swapping inputs simply involves using two different promoters sensitive to two different inputs in the proper locations. This ensures that our bacterial decoder does not just work with one specific scenario but that it will be flexible and have a wide variety of applications.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>One feature of our bacterial decoder will be that it will be modular. In other words, it <ins class="diffchange diffchange-inline">should </ins>be <ins class="diffchange diffchange-inline">trivial </ins>to change the two inputs that our decoder will respond to. Our design uses only two promoters that are each sensitive to a specific input. Swapping inputs simply involves using two different promoters sensitive to two different inputs in the proper locations. This ensures that our bacterial decoder does not just work with one specific scenario but that it will be flexible and have a wide variety of applications.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our bacterial decoder will also involve the usage of small RNAs, or sRNAs. sRNAs are very short transcripts of RNA (usually ~100 base pairs in ''E. coli'') that are used in bacteria to positively or negatively regulate genes at the translational level by binding to the mRNA of the gene and either occluding the ribosomal binding site (RBS) and preventing ribosome binding or recruiting nucleases to degrade the mRNA. The roles and mechanisms of sRNAs in bacteria currently comprise a very prominent topic in the field of biology, and many sRNAs have been extensively studied and characterized. Using sRNAs in our bacterial decoder will help us achieve on/off behavior rather than intermediate levels of outputs, since sRNAs do little at low concentrations but demonstrate strong regulation at high concentrations. They also offer other advantages over transcriptional regulation<del class="diffchange diffchange-inline">; they use </del>fewer cell resources<del class="diffchange diffchange-inline">, </del>and <del class="diffchange diffchange-inline">they </del>turn genes on or off more quickly. Currently, there are very few working sRNAs in the Parts Registry. During the course of our project, we hope to test several sRNAs and add them to the Parts Registry as working components for the use of other iGEM teams.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our bacterial decoder will also involve the usage of small <ins class="diffchange diffchange-inline">non-coding </ins>RNAs, or sRNAs. sRNAs are very short transcripts of RNA (usually ~100 base pairs in ''E. coli'') that are used in bacteria to positively or negatively regulate genes at the translational level by binding to the mRNA <ins class="diffchange diffchange-inline">transcript </ins>of the gene and either occluding the ribosomal binding site (RBS) and preventing ribosome binding or recruiting nucleases to degrade the mRNA. The roles and mechanisms of sRNAs in bacteria currently comprise a very prominent topic in the field of biology, and many sRNAs have been extensively studied and characterized. Using sRNAs in our bacterial decoder will help us achieve on/off behavior rather than intermediate levels of outputs, since sRNAs do little at low concentrations but demonstrate strong regulation at high concentrations. They also offer other advantages over transcriptional regulation <ins class="diffchange diffchange-inline">such as using </ins>fewer cell resources and <ins class="diffchange diffchange-inline">the ability to </ins>turn genes on or off more quickly. Currently, there are very few working sRNAs in the Parts Registry. During the course of our project, we hope to test several sRNAs <ins class="diffchange diffchange-inline">along with their respective target sequences </ins>and add them to the Parts Registry as working components for the use of other iGEM teams.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"></div></td></tr>
</table>Reifhttp://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&diff=94555&oldid=prevMeaganM at 20:18, 12 October 20092009-10-12T20:18:43Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:18, 12 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 32:</td>
<td colspan="2" class="diff-lineno">Line 32:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="uicontentbox"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="uicontentbox"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></html></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">== '''Presentation''' ==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Very rough drafts of the BIOE 120 introductory ppt. [[Image:Illinois_Bioe_120_(ppt).ppt]] and iGEM competition powerpoint. [[Image:IllinoisiGEM2009Presentation.ppt]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">P.S. Dave,you're welcome to change how this is set up on the website.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><html></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></div></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>MeaganMhttp://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&diff=94550&oldid=prevMeaganM: /* Presentation */2009-10-12T20:16:42Z<p><span class="autocomment">Presentation</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:16, 12 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 36:</td>
<td colspan="2" class="diff-lineno">Line 36:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Presentation''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Presentation''' ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Very rough drafts of the BIOE 120 introductory ppt. and iGEM competition powerpoint.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Very rough drafts of the BIOE 120 introductory ppt. <ins class="diffchange diffchange-inline">[[Image:Illinois_Bioe_120_(ppt).ppt]] </ins>and iGEM competition powerpoint. <ins class="diffchange diffchange-inline">[[Image:IllinoisiGEM2009Presentation.ppt]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>P.S. Dave,you're welcome to change how this is set up on the website.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>P.S. Dave,you're welcome to change how this is set up on the website.</div></td></tr>
</table>MeaganMhttp://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&diff=94535&oldid=prevMeaganM at 20:10, 12 October 20092009-10-12T20:10:11Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:10, 12 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 32:</td>
<td colspan="2" class="diff-lineno">Line 32:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="uicontentbox"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="uicontentbox"></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></html></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">== '''Presentation''' ==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Very rough drafts of the BIOE 120 introductory ppt. and iGEM competition powerpoint.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">P.S. Dave,you're welcome to change how this is set up on the website.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><html></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></div></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>MeaganMhttp://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&diff=88730&oldid=prevKorench1: /* Introduction */2009-10-09T01:27:19Z<p><span class="autocomment">Introduction</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:27, 9 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 15:</td>
<td colspan="2" class="diff-lineno">Line 15:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Srna.jpg|thumb|sRNAs can be either ''cis''(A) or ''trans''(B). They can prevent protein synthesis from an mRNA by several methods, including binding to the RBS and preventing ribosome binding or binding to the 5' UTR and recruiting degradative enzymes. Some sRNAs positively regulate a gene by binding to a folded mRNA and revealing the RBS.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Srna.jpg|thumb|sRNAs can be either ''cis''(A) or ''trans''(B). They can prevent protein synthesis from an mRNA by several methods, including binding to the RBS and preventing ribosome binding or binding to the 5' UTR and recruiting degradative enzymes. Some sRNAs positively regulate a gene by binding to a folded mRNA and revealing the RBS.]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our team has chosen to create a 2-to-4 binary decoder in ''Escherichia coli''. Our novel bacteria will be able to sense concentrations of two inputs (such as two different sugars), identify these concentrations as "on" or "off", and produce one of four unique outputs (fluorescent proteins) depending on the combination of inputs. Thus, our four outputs can be represented in terms of our binary inputs as 00, 01, 10, and 11, where 0 corresponds to the "off" state (low concentration), 1 corresponds to the "on" state (high concentration), the first digit corresponds to the state of <del class="diffchange diffchange-inline">lactose</del>, and the second digit corresponds to the state of <del class="diffchange diffchange-inline">arabinose</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our team has chosen to create a 2-to-4 binary decoder in ''Escherichia coli''. Our novel bacteria will be able to sense concentrations of two inputs (such as two different sugars), identify these concentrations as "on" or "off", and produce one of four unique outputs (fluorescent proteins) depending on the combination of inputs. Thus, our four outputs can be represented in terms of our binary inputs as 00, 01, 10, and 11, where 0 corresponds to the "off" state (low concentration), 1 corresponds to the "on" state (high concentration), the first digit corresponds to the state of <ins class="diffchange diffchange-inline">the first input</ins>, and the second digit corresponds to the state of <ins class="diffchange diffchange-inline">the second input</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Korench1http://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&diff=88728&oldid=prevKorench1: /* Introduction */2009-10-09T01:26:08Z<p><span class="autocomment">Introduction</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:26, 9 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 15:</td>
<td colspan="2" class="diff-lineno">Line 15:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Srna.jpg|thumb|sRNAs can be either ''cis''(A) or ''trans''(B). They can prevent protein synthesis from an mRNA by several methods, including binding to the RBS and preventing ribosome binding or binding to the 5' UTR and recruiting degradative enzymes. Some sRNAs positively regulate a gene by binding to a folded mRNA and revealing the RBS.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Srna.jpg|thumb|sRNAs can be either ''cis''(A) or ''trans''(B). They can prevent protein synthesis from an mRNA by several methods, including binding to the RBS and preventing ribosome binding or binding to the 5' UTR and recruiting degradative enzymes. Some sRNAs positively regulate a gene by binding to a folded mRNA and revealing the RBS.]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our team has chosen to create a 2-to-4 binary decoder in ''Escherichia coli''. Our novel bacteria will be able to sense concentrations of two inputs (<del class="diffchange diffchange-inline">lactose and arabinose</del>), identify these concentrations as "on" or "off", and produce one of four unique outputs (fluorescent proteins) depending on the combination of inputs. Thus, our four outputs can be represented in terms of our binary inputs as 00, 01, 10, and 11, where 0 corresponds to the "off" state (low concentration), 1 corresponds to the "on" state (high concentration), the first digit corresponds to the state of lactose, and the second digit corresponds to the state of arabinose.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our team has chosen to create a 2-to-4 binary decoder in ''Escherichia coli''. Our novel bacteria will be able to sense concentrations of two inputs (<ins class="diffchange diffchange-inline">such as two different sugars</ins>), identify these concentrations as "on" or "off", and produce one of four unique outputs (fluorescent proteins) depending on the combination of inputs. Thus, our four outputs can be represented in terms of our binary inputs as 00, 01, 10, and 11, where 0 corresponds to the "off" state (low concentration), 1 corresponds to the "on" state (high concentration), the first digit corresponds to the state of lactose, and the second digit corresponds to the state of arabinose.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 33:</td>
<td colspan="2" class="diff-lineno">Line 33:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="uicontentbox"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="uicontentbox"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Sources''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Sources''' ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Korench1http://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&diff=30461&oldid=prevKorench1 at 16:31, 28 July 20092009-07-28T16:31:11Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 16:31, 28 July 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Illinois}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Illinois}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{IllProjNav}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{IllProjNav}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><html></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><!-- I moved this off of the template since I thought it looked better here, but it covers up the menus... --></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><p align="center"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><object width="550" height="200"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><param name="movie" value="ecoli.swf"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><embed src="/wiki/images/a/a7/Ecoli.swf" width="550" height="200" wmode="transparent"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></embed></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></object></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></p></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></html></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="uicontentbox"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="uicontentbox"></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 35:</td>
<td colspan="2" class="diff-lineno">Line 23:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our bacterial decoder will also involve the usage of small RNAs, or sRNAs. sRNAs are very short transcripts of RNA (usually ~100 base pairs in ''E. coli'') that are used in bacteria to positively or negatively regulate genes at the translational level by binding to the mRNA of the gene and either occluding the ribosomal binding site (RBS) and preventing ribosome binding or recruiting nucleases to degrade the mRNA. The roles and mechanisms of sRNAs in bacteria currently comprise a very prominent topic in the field of biology, and many sRNAs have been extensively studied and characterized. Using sRNAs in our bacterial decoder will help us achieve on/off behavior rather than intermediate levels of outputs, since sRNAs do little at low concentrations but demonstrate strong regulation at high concentrations. They also offer other advantages over transcriptional regulation; they use fewer cell resources, and they turn genes on or off more quickly. Currently, there are very few working sRNAs in the Parts Registry. During the course of our project, we hope to test several sRNAs and add them to the Parts Registry as working components for the use of other iGEM teams.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our bacterial decoder will also involve the usage of small RNAs, or sRNAs. sRNAs are very short transcripts of RNA (usually ~100 base pairs in ''E. coli'') that are used in bacteria to positively or negatively regulate genes at the translational level by binding to the mRNA of the gene and either occluding the ribosomal binding site (RBS) and preventing ribosome binding or recruiting nucleases to degrade the mRNA. The roles and mechanisms of sRNAs in bacteria currently comprise a very prominent topic in the field of biology, and many sRNAs have been extensively studied and characterized. Using sRNAs in our bacterial decoder will help us achieve on/off behavior rather than intermediate levels of outputs, since sRNAs do little at low concentrations but demonstrate strong regulation at high concentrations. They also offer other advantages over transcriptional regulation; they use fewer cell resources, and they turn genes on or off more quickly. Currently, there are very few working sRNAs in the Parts Registry. During the course of our project, we hope to test several sRNAs and add them to the Parts Registry as working components for the use of other iGEM teams.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p align="center"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><object width="550" height="200"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><param name="movie" value="ecoli.swf"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><embed src="/wiki/images/a/a7/Ecoli.swf" width="550" height="200" wmode="transparent"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></embed></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></object></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></html></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><html></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="uicontentbox"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="uicontentbox"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td></tr>
</table>Korench1http://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&diff=22344&oldid=prevGraham at 00:01, 18 July 20092009-07-18T00:01:49Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:01, 18 July 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 38:</td>
<td colspan="2" class="diff-lineno">Line 38:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><html></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div id="uicontentbox"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></html></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Sources''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Sources''' ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 47:</td>
<td colspan="2" class="diff-lineno">Line 50:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Wikipedia. "Decoder". http://en.wikipedia.org/wiki/Decoder.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Wikipedia. "Decoder". http://en.wikipedia.org/wiki/Decoder.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><html></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></div></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></html></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{IllinoisBottomNav}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{IllinoisBottomNav}}</div></td></tr>
</table>Grahamhttp://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&diff=22343&oldid=prevGraham: /* Introduction */2009-07-18T00:01:21Z<p><span class="autocomment">Introduction</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:01, 18 July 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 14:</td>
<td colspan="2" class="diff-lineno">Line 14:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><html></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div id="uicontentbox"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></html></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Introduction''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Introduction''' ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 31:</td>
<td colspan="2" class="diff-lineno">Line 34:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our bacterial decoder will also involve the usage of small RNAs, or sRNAs. sRNAs are very short transcripts of RNA (usually ~100 base pairs in ''E. coli'') that are used in bacteria to positively or negatively regulate genes at the translational level by binding to the mRNA of the gene and either occluding the ribosomal binding site (RBS) and preventing ribosome binding or recruiting nucleases to degrade the mRNA. The roles and mechanisms of sRNAs in bacteria currently comprise a very prominent topic in the field of biology, and many sRNAs have been extensively studied and characterized. Using sRNAs in our bacterial decoder will help us achieve on/off behavior rather than intermediate levels of outputs, since sRNAs do little at low concentrations but demonstrate strong regulation at high concentrations. They also offer other advantages over transcriptional regulation; they use fewer cell resources, and they turn genes on or off more quickly. Currently, there are very few working sRNAs in the Parts Registry. During the course of our project, we hope to test several sRNAs and add them to the Parts Registry as working components for the use of other iGEM teams.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our bacterial decoder will also involve the usage of small RNAs, or sRNAs. sRNAs are very short transcripts of RNA (usually ~100 base pairs in ''E. coli'') that are used in bacteria to positively or negatively regulate genes at the translational level by binding to the mRNA of the gene and either occluding the ribosomal binding site (RBS) and preventing ribosome binding or recruiting nucleases to degrade the mRNA. The roles and mechanisms of sRNAs in bacteria currently comprise a very prominent topic in the field of biology, and many sRNAs have been extensively studied and characterized. Using sRNAs in our bacterial decoder will help us achieve on/off behavior rather than intermediate levels of outputs, since sRNAs do little at low concentrations but demonstrate strong regulation at high concentrations. They also offer other advantages over transcriptional regulation; they use fewer cell resources, and they turn genes on or off more quickly. Currently, there are very few working sRNAs in the Parts Registry. During the course of our project, we hope to test several sRNAs and add them to the Parts Registry as working components for the use of other iGEM teams.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><html></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></div></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></html></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Sources''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Sources''' ==</div></td></tr>
</table>Grahamhttp://2009.igem.org/wiki/index.php?title=Team:Illinois/Project&diff=20297&oldid=prevReif at 18:54, 15 July 20092009-07-15T18:54:12Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:54, 15 July 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 3:</td>
<td colspan="2" class="diff-lineno">Line 3:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{IllProjNav}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{IllProjNav}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><html></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><!-- I moved this off of the template since I thought it looked better here, but it covers up the menus... --></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p align="center"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><object width="550" height="200"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><param name="movie" value="ecoli.swf"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><embed src="/wiki/images/a/a7/Ecoli.swf" width="550" height="200" wmode="transparent"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></embed></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></object></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></html></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Introduction''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Introduction''' ==</div></td></tr>
</table>Reif