Team:Illinois/Protocols

From 2009.igem.org

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=='''Suggested Reading'''==
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[http://cda.currentprotocols.com/WileyCDA/CPTitle/isbn-047150338X,descCd-tableOfContents.html '''Current Protocols in Molecular Biology''']
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* A really good resource for explaining protocols and explaining the importance of the steps.
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* Free online
Questions about our Wiki page?  Please email Dave Korenchan at [mailto:korench1@illinois.edu korench1@illinois.edu].
Questions about our Wiki page?  Please email Dave Korenchan at [mailto:korench1@illinois.edu korench1@illinois.edu].

Revision as of 16:29, 11 July 2009

Click to go to the Illinois home page





Contents

Protocols

This page describes protocols or includes links to protocols used in our project. Recipes used are also listed in a separate section. Protocols are organized by category.

Standard

sRNA Characterization

Taken from: A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo: Johannes H. Urban and Jörg Vogel, 2009

This protocol is used to characterize an E. coli small RNA and its ability to downregulate a reporter gene (GFP). The small RNA is cloned out of the E. coli chromosome and inserted into a high-copy number plasmid (pJU-334). The sRNA's target sequence is also cloned out of the E. coli chromosome and inserted into a low-copy number plasmid (pXG-10). Both plasmids are then transformed into E. coli Top10F' cells. Successful sRNA repression is indicated by a decreased level of fluorescence in the cells.

sRNA characterization procedure

Recipes

LB Broth

LB Plates

Agarose Gels

Suggested Reading

Current Protocols in Molecular Biology

  • A really good resource for explaining protocols and explaining the importance of the steps.
  • Free online


Questions about our Wiki page? Please email Dave Korenchan at korench1@illinois.edu.