Team:Imperial College London/Drylab

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<html><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction"><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html>&nbsp; <b><i>About Autoinduction</i></b><br><br>
<html><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction"><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html>&nbsp; <b><i>About Autoinduction</i></b><br><br>
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===Protein Production===
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Based on the module 1 genetic circuit, a LacI-IPTG inducible promoter is responsible for kickstarting the production of the drug.
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* In the absence of IPTG, LacI represses the production of the drug (Cellulase or PAH)
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* When IPTG is introduced, the LacI repressing pathway is “de-repressed”, and some output protein is produced.
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[[Image:II09_NoIPTG_yesIPTG.jpg|350px|right]]
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<!-- [[Image:m1.fgc.JPG | 400px.jpg]]-->
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====Our goals====
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The modelling aims to provide an overview and better understanding of the M1 system’s function  by:
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*Characterizing the system.
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*Modeling to account for several factors that may reduce/hinder the production of  the protein drug such as:
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**Lac promoter leakiness
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**IPTG toxicity
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**Stability of output protein
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 +
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This module is an integral part of the design, as large-scale commercialization of the drug of interest depends on finding the optimal conditions for protein production. We implemented a system of differential equations, having made some assumptions and predictions about how the system will behave.
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<html><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Protein_production/Analysis"><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html>&nbsp; <b><i>About the model assumptions and predictions!
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</i></b><br><br>
<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Genetic_Circuit"><img width=150px src="https://static.igem.org/mediawiki/2009/d/d9/II09_Temp_ArrowLeft.png"></a>
<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Genetic_Circuit"><img width=150px src="https://static.igem.org/mediawiki/2009/d/d9/II09_Temp_ArrowLeft.png"></a>

Revision as of 12:36, 17 October 2009

Contents

II09 DryLabThumb.pngDry Lab Hub

Welcome to the Dry Lab!

The goal of the Dry Lab has been to support the Wet Lab by answering questions of interest. Conclusions from the models aim to provide the team with a functional understanding of the system as well as design considerations that should be addressed in the genetic constructs.

In the Dry Lab, we have implemented several models, explaining the behaviour of different parts of the system. This section will use provide a summary of the models. The conclusions drawn can also be found here.

Modelling in this project has been important in defining the "Engineering constraints" of the project, and in particular, we have focused in quantifiying the temporal control aspects of our system. This page provides an overview of the models we have implemented.

Autoinduction

II09 diauxi illust.jpg

Starting the encapsulation process is related to diauxic growth of bacteria under the presence of two carbon sources. 2 models from literature have been discussed and implemented in this section. More information about each can be found in the extra tabs. After analysis of the models, we picked one of them as the best representation for our data. The sections below will provide a summary about each model, and at the end, discuss which one was picked and why. The diauxic growth phenomenon is tricky to understand, and the models we have chosen illustrate different approaches that groups have taken to explain what happens when bacteria switch to feeding from a primary carbon source to a secondary carbon source.

Goal of the models

  • Provide understanding of the diauxic growth phenomenon
  • Find out the time taken to consume glucose in the medium to characterize the CRP promoter.

  About Autoinduction

Protein Production

Based on the module 1 genetic circuit, a LacI-IPTG inducible promoter is responsible for kickstarting the production of the drug.

  • In the absence of IPTG, LacI represses the production of the drug (Cellulase or PAH)
  • When IPTG is introduced, the LacI repressing pathway is “de-repressed”, and some output protein is produced.
II09 NoIPTG yesIPTG.jpg


Our goals

The modelling aims to provide an overview and better understanding of the M1 system’s function by:

  • Characterizing the system.
  • Modeling to account for several factors that may reduce/hinder the production of the protein drug such as:
    • Lac promoter leakiness
    • IPTG toxicity
    • Stability of output protein


This module is an integral part of the design, as large-scale commercialization of the drug of interest depends on finding the optimal conditions for protein production. We implemented a system of differential equations, having made some assumptions and predictions about how the system will behave.

  About the model assumptions and predictions!


Click on the links to find out more!

 
Autoinduction
Protein Production
Drug Kinetics Genome Deletion



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