Team:Imperial College London/M3/RestrictionEnzymes

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II09 Thumb m3.pngModule 3: Genome Deletion Overview

Restriction Enzymes

After Module 2 has been completed, genome deletion is triggered by raising the temperature.
see thermoinduction under temporal control)

The cell then produces the restriction enzymes DpnII and TaqI.

Restriction enzymes DpnII and TaqI specifically target and cut short 4 base DNA sequences. With a high frequency of cutting, the genetic material contained within the cell will all be destroyed, including any inserted DNA.

II09 cut dna.jpg

A distinct advantage of using restriction enzymes for our 'killing' mechanism is that the cell membrane is left intact afterwards, and the protein of interest will still be protected by the encapsulated cell. This renders the bacterium no more than an inanimate shell containing our protein drug of choice.

Inanimate shell.jpg




  about the restriction enzymes TaqI and DpnII.



  About the ethical implications of live organisms.




Module 3 - Genome Deletion

Genetic Circuit
WetLab
Modelling
Results

Mr. Gene   Geneart   Clontech   Giant Microbes