Team:Imperial College London/M3/RestrictionEnzymes

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II09 Thumb m3.pngModule 3: Genome Deletion Overview

Restriction Enzymes

In our system, the restriction enzymes DpnII and TaqI are produced.

These restriction enzymes will cleave DNA at recognition sites. This leads to a double-stranded breakage in DNA, which will subsequently result in cell death unless repair is performed in time.

These are 4 base cutters, specifically targetting and cutting the sequences GATC and TCGA. With a high frequency of cutting, the repair system would not be able to cope with multiple cleavages, so the genetic material contained within the cell will all be destroyed, including any inserted DNA.

II09 taq digestion.jpg


A distinct advantage of using restriction enzymes for our 'killing' mechanism is that the cell membrane is left intact afterwards, and the protein of interest will still be protected by the encapsulated cell. This renders the bacterium no more than an inanimate shell containing our protein drug of choice.

Inanimate shell.jpg




  about the restriction enzymes TaqI and DpnII.



  About the ethical implications of live organisms.




Module 3 - Genome Deletion

Genetic Circuit
WetLab
Modelling
Results

Mr. Gene   Geneart   Clontech   Giant Microbes