"
Team:Johns Hopkins-BAG/A New Standard
From 2009.igem.org
(→Someething) |
(→Someething) |
||
| Line 1: | Line 1: | ||
{{Template:JHU_BAG_Top}} | {{Template:JHU_BAG_Top}} | ||
{{Template:JHU_BAG_SideBar}} | {{Template:JHU_BAG_SideBar}} | ||
| - | == | + | ==Building Blocks: a revolution in Large DNA/Genome assembly== |
| - | The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. Furthermore, current Biobrick construction standards [ | + | The Johns Hopkins University Build a Genome team has proposed a new standard for the construction and assembly of large DNA. The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. Furthermore, current Biobrick construction standards [RFC10, RFC11,RFC12 etc.] rely heavily on restriction enzyme based methods, which can be sequence, cost and time restrictive. This is especially true of large DNA assembly, where 100% control of over DNA sequence may be mandatory, such as in whole genome assembly. We propose a novel standard, The Building Block Method, for both the construction of standard parts and their assembly |
This Building Block standard can be either: | This Building Block standard can be either: | ||
Revision as of 17:47, 12 October 2009
Building Blocks: a revolution in Large DNA/Genome assembly
The Johns Hopkins University Build a Genome team has proposed a new standard for the construction and assembly of large DNA. The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. Furthermore, current Biobrick construction standards [RFC10, RFC11,RFC12 etc.] rely heavily on restriction enzyme based methods, which can be sequence, cost and time restrictive. This is especially true of large DNA assembly, where 100% control of over DNA sequence may be mandatory, such as in whole genome assembly. We propose a novel standard, The Building Block Method, for both the construction of standard parts and their assembly
This Building Block standard can be either:
A) an interchangeable or B) a non-interchangeable format, based on the desired use of the part.
