Team:Johns Hopkins-BAG/Synthetic Yeast Genome

From 2009.igem.org

(Difference between revisions)
(Synthetic Yeast Genome)
(Genome Stabilization)
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==Genome Stabilization==
==Genome Stabilization==
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===tRNA Array==
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tRNA genes have been show to be hotspots for DNA instability due to several reasons:
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*Target of retrotransposon incorporation.
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*Transcribed heavily, which can lead to single strand breaks during DNA synthesis, when RNA polymerase III and DNA polymerase collide.
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*Homologous regions upstream of tRNA genes, due to retrotransposon insertions, can aid homologous recombination of chromosomal regions
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Thus in the yeast 2.0 genome all tRNA genes will be moved to their own chromosome, so that their absence throughout the rest of the genome can be observed. All introns will also be removed from tDNA, as it is another goal of the project to observed the effect of a yeast genome with less introns. Duplicate genes will also be removed.
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''rox'' Recombination sites will be places between each tRNA gene, which will be used to observe if there is a preference for tRNA gene relative location and copy number
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==Genome Destabilization==
==Genome Destabilization==

Revision as of 16:59, 19 October 2009

Contents

Synthetic Yeast Genome

Our iGEM team originated from participants in the Synthetic Yeast Genome Project, which is an ongoing effort to redesign and construct the Saccharomyces cerevisiae genome. Many alternations to the genome have been made in the new design including: removal of transposable element, relocation of tRNA genes, removal of "unnecessary genes", and incorporation of site-specific recombination sites. More details about the project can be found on the Synthetic Yeast Genome Wiki

The team members on our team focus on the the parts of the project that invesgiates the role of genome stability in the yeast genome.

Genome Stabilization

=tRNA Array

tRNA genes have been show to be hotspots for DNA instability due to several reasons:

  • Target of retrotransposon incorporation.
  • Transcribed heavily, which can lead to single strand breaks during DNA synthesis, when RNA polymerase III and DNA polymerase collide.
  • Homologous regions upstream of tRNA genes, due to retrotransposon insertions, can aid homologous recombination of chromosomal regions

Thus in the yeast 2.0 genome all tRNA genes will be moved to their own chromosome, so that their absence throughout the rest of the genome can be observed. All introns will also be removed from tDNA, as it is another goal of the project to observed the effect of a yeast genome with less introns. Duplicate genes will also be removed.

rox Recombination sites will be places between each tRNA gene, which will be used to observe if there is a preference for tRNA gene relative location and copy number

Genome Destabilization