Team:KULeuven/11 August 2009

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Latest revision as of 08:46, 10 September 2009


Progress of parts

[edit] Blue Light Receptor

  1. Restriction digest
    • cut with EcoRI and SpeI, incubation for 1,5 hour
  2. Gel electrophoresis of
    • The cut piece should be 109 bp
  3. Restriction digest
  4. Electroporation of parts - and in electrocompetent cells

[edit] Vanillin Production

  • There was no growth of sam8/sam5, fcs/ech and COMT plates.
    • Possible reason is a problem with the electrocompetent cells
    • Or the concentration of sam5/sam8 is too low
  • Plan for tomorrow is to create new electrocompetent cells
    • Prepared LB and glycerol today
  • Made liquid cultures sam8, sam5, fcs and ech
  • Made new agarplates with Ampicilin

[edit] Vanillin Receptor

  • We also received another strain of Agrobacterium. We brought this new strain in culture.
  • To make things clear the genes from the first Agrobacterium will from now on be called A,B,G and R for respectively virA, promotor region virB , virG and rpoA. For the second Agrobacterium we will use W,X,Y and Z to indicate the same genes.
  • We achieved good results for genes B,G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampicillin. We isolated the good colonies again.
  • There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heatshock all over for A.

[edit] Key/Lock/Anti-Key