Team:KULeuven/11 September 2009


Project progress

Progress of parts

[edit] Blue Light Receptor

  1. The electroporated ligY did not grow. A new restriction digest on pcr product was performed with EcoRI and PstI.This was put on gel. There was a good signal so it was used to perform a ligation with vector pSB1A2.
  2. A liquid culture that was made from pSB3K3 a few days ago showed growth after all. This was miniprepped and restriction digested to check whether the vector is actually present.
  3. The liquid cultures with our blue light construct (lig) that stood overnight at 16°C was FACS-ed. Half of the cultures were exposed to blue light during the day and put under the FACS in the evening.

[edit] Vanillin Production

  • Miniprep SAMS
part concentration 260/280 260/230
SAMS1 1 118,2
SAMS1 2 105,2
SAMS1 3 95,7
SAMS1 4 112,0
SAMS2 1 67,8
SAMS2 2 122,6
SAMS2 3 90,0
SAMS2 4 99,6
  • Restriction SAMS + Promotor (
    • Promotor restriction results in 2 parts of 800 bp and 2k bp
  • EFT electroporation no result, try again on monday...

[edit] Vanillin Receptor

  • Glycerolstock was made for virG and a sample was send for seqencing
  • virA was ented
  • new TOPOcloning was done for rpoA with a new protocol we recieved

[edit] Key/Lock/Anti-Key