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Team:KULeuven/4 August 2009
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Revision as of 09:02, 4 September 2009
Project progress
Progress of parts
[edit] Blue Light Receptor
[edit] Vanillin Production
Liquid cultures were made out of the sam5, sam8 and COMT colonies grown overnight. They will be mini-prepped tomorrow to get the plasmid DNA out.
Plasmid DNA from the ech and fcs liquid cultures was isolated today. It yielded respectively 108,8 and 113,9 ng/µl.
[edit] Vanillin Receptor
- The primers made for the directed mutagenesis on Friday were ordered
- PCR was done with primers for virA, virG, promotorregion virB and RpoA with template DNA from Agrobacterium tumefaciens pTiA6NC/A1011 to isolate the genes
