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Essencia Coli

Schematic illustration of the setup for controlling concetration of molecule 'X'

{ text-align:justify } The main idea is to synthesize Vanillin and controll the synthesis such that the concentration of Vanillin stays constant. The value of this concentration can be set by intensity if blue light. This concept however can be extended to other molecules that need to be kept at a certain concentration, this can be for example other odors or flavours or something completely different like essential nutrients or vitamins. In the figure on the left an illustration of the controlling mechanism is explained. In here Vanillin is changed to molecule 'X' because of it's universal charachter. In the figure the cell synthesizes molecule 'X' and at the same time measures the concentration of this molecule outside the cell and uses this measurement to controll the synthesis and keep the concentration outside the cell constant. This concentration can then be modulated using light intensity.

For proof of concept the molecule 'X' is set to be vanillin, to extend this principle to other molecule all that is needed is a concentration dependent receptor. The light receptor will activate transcription of A on irradiation of blue light. This will stimulate synthesis of Vanillin. The concentration vanillin outside the cell is measured with a vanillin receptor. The activation of the vanillin receptor will result in transcription of B and block the actions of A. The activation of synthesis by A is done with a key/lock setup. The translation of the Vanillin synthesizing proteins need a key in order to translate, A will provide the key. Therefore upon irradiation of blue light the required proteins will be translated. The inhibition of A by B is done by translating an anti-sense mRNA of A, blocking the actions of the key. Thus, if the concentration of vanillin increases, the synthesis of vanillin-synthesizing proteins will decrease untill it stops completely.

The complete system with the corresponding biobricks can be seen below, as well as the biobricks which still need to be created from the genes (blue light receptor and vanillin synthesis)

Overview of the system with corresponding biobricks

Biobricks

all the parts we are planning on using/making:

Ribokey controlled by blue light receptor

the ribokey controlled by the blue light receptor

Vanillasynthesis with ribolock

Vanillinsynthesis with ribolock in front.

Vanillin receptor

Antikey

Antikey that is produced when the vanillin concentration reaches high levels.

to be constructed

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