Team:KULeuven/Abstract

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(Essencia Coli)
(Essencia Coli)
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The main idea is to synthesize a molecule 'X' and at the same time measure the concentration of this molecule outside the cell in order to keep the concentration constant. This concentration can then be modulated using light intensity. This creates a controll system with feedback from the concentration.
The main idea is to synthesize a molecule 'X' and at the same time measure the concentration of this molecule outside the cell in order to keep the concentration constant. This concentration can then be modulated using light intensity. This creates a controll system with feedback from the concentration.
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For proof of concept the molecule 'X' is set to be vanillin. Vanillin will be synthesized and can freely diffuse out of the cell. The concentration outside the cell is measured with a vanillin receptor.  
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For proof of concept the molecule 'X' is set to be vanillin. The light receptor will synthesize molecule B on irradiation of bleu light.Vanillin will be synthesized and can freely diffuse out of the cell. The concentration outside the cell is measured with a vanillin receptor.  
   
   
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how we'll control the concentration
how we'll control the concentration
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=Biobricks=
=Biobricks=
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Revision as of 14:31, 15 July 2009

Essencia Coli

Overview of the system with corresponding biobricks

The main idea is to synthesize a molecule 'X' and at the same time measure the concentration of this molecule outside the cell in order to keep the concentration constant. This concentration can then be modulated using light intensity. This creates a controll system with feedback from the concentration.

For proof of concept the molecule 'X' is set to be vanillin. The light receptor will synthesize molecule B on irradiation of bleu light.Vanillin will be synthesized and can freely diffuse out of the cell. The concentration outside the cell is measured with a vanillin receptor.


Mssbluevanilla.jpg how we'll control the concentration

Biobricks

all the parts we are planning on using/making:

Key.png

the ribokey controlled by the blue light receptor

Synthese.png

Vanillinsynthesis with ribolock in front.

Receptor.png

Vanillin receptor.

Antikey.png

Antikey that is produced when the vanillin concentration reaches high levels.

to be constructed